Jeremy Leitz4, Jiangang Long3, Jiankang Liu3, Dapeng Yu1,2 & Qing Zhao1,2 α-Synuclein (α-Syn) is an abundant cytosolic protein involved in the release of neurotransmitters in presynaptic terminal and its aberrant aggregation is found to be associated with Parkinson's disease. ...
Fig. 1: Principle and performance of dynamic mass photometry. a, Schematic diagram of dynamic mass photometry of protein complexes diffusing on an SLB. The images were acquired at 331 Hz and processed with a sliding median filter, which showed individual protein complexes on the bilayer as dif...
1a). Previous research reported the design of a bispecific fusion protein consisting of m36.4, a single-domain antibody targeting the CD4i coreceptor-binding site, and mD1.22, a mutant of the CD4 D1 domain targeting the CD4-binding site, which, by recognizing the HIV-1 Env protein with ...
To further demonstrate that the increased cleavage is because of a direct cleavage by BACE1 and not merely because of secondary effects, the epitope-tagged proteins were immunoprecipitated from the cell lysates using an antibody against the HA-tag and subject to an in vitro cleavage assay by purif...
Schematic diagram of the mechanisms by which TGF-β1 attenuates rosiglitazone-induced adipogenic differentiation in OP9-DL1 cells. TGF-β1 increases the recruitment of Smad2/3 and HDAC1 and reduces the level of H3K14ac at the promoter region of the PPARγ gene, thereby transcriptionally down-reg...
In the era of single-cell sequencing, there is a growing need to extract insights from data with clustering methods. Here, we introduce Forest Fire Clustering, an efficient and interpretable method for cell-type discovery from single-cell data. Forest Fi
Also, according to this method, a secondary antibody encoded with a fluorescent material generally needs to be used, and thus, analysis may become complicated and related costs tend to increase. As illustrated in FIG. 11, in the optic fiber microbead array, microbeads are encoded with a ...
Results The structure of the novel array electrode. The novel array electrode for multi-spots assay was designed and the schematic diagram of its structure is shown in Fig. 1. One pair of the counter and reference electrodes (a platinum counter electrode, an Ag/AgCl reference electrode) are ...
A central effort of today’s neuroscience is to study the brain’s ’wiring diagram’. The nervous system is believed to be a network of neurons interacting with each other through synaptic connection between axons and dendrites, therefore the neuronal connectivity map not only depicts the underlyi...
FIG. 15 is a cross-sectional diagram of an inorganic DFB laser sensor chip described below as Example 7. FIGS. 16A-16D are perspective views, partially in cross-section, of different types of one dimensional gratings which can used for the DFB laser sensor. FIG. 17 is an illustration of ...