首先,我们可以把一个细胞的周期分为间期interphase以及分裂期celldivision phase. 其中interphase是在做一些准备工作,而celldivision phase则是真正的在分裂 当然,事情并没有这么简单。 每一个phase接下来都可以更细节的分为几个阶段。 Interphase G0/G1phase -A resting phase -Cell perform own function -Some type...
IL-12-dependent innate immunity arrests endothelial cells in G0-G1 phase by a p21(Cip1/Waf1)-mediated mechanism.AngiogenesisCell proliferationCytokinesSignal transductionT cellsan inhibitor of cyclin kinases. This effect requires the activation of STAT1, PKC未 and p38 MAPK, while p53 is ineffective...
3.外周血单个核细胞的分离、培养和分选:采用Ficoll密度梯度离心法分离患者外周血单个核细胞(peripheral blood mononuclear cell,PBMC),按每孔5×105个细胞加入96孔板中,加入HBsAg肽库(工作浓度为5 μg/mL)进行刺激培养,并加CD28、白...
采用实时荧光定量聚合酶链反应(real-time fluorescence quantitative polymerase chain reaction,RTFQ-PCR)检测CHKA基因mRNA的表达水平,采用细胞计数试剂盒-8(cell counting kit-8,CCK-8)法检测细胞增殖能力,采用流式细胞术检测细胞周期和凋亡,...
Here we show that the fission yeast homologue Rad3 and the human ATR regulate events also in G1 phase in an unperturbed cell cycle. Rad3Δ mutants or human cells exposed to ATR inhibitor in G1 enter S phase prematurely, which results in increased DNA damage. Furthermore, ATR inhibition ...
2015年初,李国红课题组的前期研究发现了CENP-A分子的Ser68的磷酸化/去磷酸化参与调节细胞G1期CENP-A在着丝粒区的装配(Yu et al., Development Cell, 2015),清楚地揭示了CENP-A如何装配到着丝粒区。 在以上研究的基础上,李国红课题组又对CENP-A装配到着丝粒区后如何调节染色质结构及发挥着丝粒功能进行了重点研究。
Owa T, Yoshino H, Yoshimatsu K, Nagasu T (2001), Cell cycle regulation in G1 phase: A promising target for the development of new chemotherapeutic anticancer agents, Curr. Med. Chem. 8: 1487-1503Owa T, Yoshino H, Yoshimatsu K, Nagasu T. Cell cycle regulation in the G1 phase: a ...
In our screen, HJURP is not a prominent contributor to overall CENP-A maintenance, relative to SENP6 or other candidate maintenance factors (Supplementary Data 2). Nevertheless, S phase may be a critical cell cycle window where CENP-A maintenance requires the re-assembly of pre-existing CENP-...
By analyzing the timing of death, cell cycle markers, BrdU-labeled S phase cells, and phase-specific centrifugally elutriated cultures from stimulated Jurkat T cells and peripheral blood lymphocytes, we found that AID occurs from a late G1 check point prior to activation of cyclin E:Cdk2 ...
Transfer these cells to the centrifuge tube from Step 1. Centrifuge at 125 x g for 5 to 10 minutes. Remove medium and resuspend pellet in fresh complete medium. Add appropriate aliquots of cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation ratio: A ...