Boster’s SDS PAGE Sample Buffer 5X (Reducing) is the most commonly used sample buffer for Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis (SDS-PAGE) of denatured proteins in the Laemmli SDS-PAGE system. This buffer is very important in the preparation of protein samples and loading ...
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Brain lysates (30 µg) were reduced in Laemmli buffer (15 min at 65 °C), then loaded and separated on 10% SDS-PAGE. Resolved proteins were stained with Coomassie Brilliant Blue G-250 (0.5%w/vin 40% aqueous methanol and 10% glacial acetic acid) overnight at 4 °C. Gels were de...