非变性非还原性蛋白上样缓冲液(Native Gel Sample Loading Buffer,5X),是一种经过改良的以溴酚蓝为染料,5倍浓缩的蛋白上样缓冲液。 本产品中不含SDS等变性试剂,也不含DTT、巯基乙醇等还原性试剂。 可以用于常规的非变性非还原性聚丙烯酰胺凝胶(PAGE)蛋白样品的上样。
Boster’s SDS PAGE Sample Buffer 5X (Reducing) is the most commonly used sample buffer for Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis (SDS-PAGE) of denatured proteins in the Laemmli SDS-PAGE system. This buffer is very important in the preparation of protein samples and loading ...
Laemmli’s (SDS-Sample) Buffer (Laemmli(SDS样品)缓冲液)reducing,4X 公司简介 上海森兴研生物技术有限公司是一家集研发,销售为一体的生物公司,公司目前可以提供ELISA试剂盒,生化试剂盒,PCR试剂盒,生化试剂、分子生物学试剂及试剂盒,各种及免疫学试剂盒、标准品,实验耗材等多门类上万种产品,产品涉及分子生物学、细...
For detection of PS1 NT, samples were mixed with standard Laemmli buffer but not boiled prior to electrophoresis (22). Membranes were Ponceau S–stained immediately after transfer and then probed overnight at 4 °C or for 1 h at room temperature (TuJ1) with primary antibody under the ...
Brain lysates (30 µg) were reduced in Laemmli buffer (15 min at 65 °C), then loaded and separated on 10% SDS-PAGE. Resolved proteins were stained with Coomassie Brilliant Blue G-250 (0.5%w/vin 40% aqueous methanol and 10% glacial acetic acid) overnight at 4 °C. Gels were de...