Thermo Scientific 溶菌酶是一种酶,其特征在于能够破坏细菌细胞壁以提高蛋白或核酸提取效率。甘油冻存溶菌酶的特点:•细菌细胞壁溶解酶可改善包涵体蛋白纯化•与 Thermo Scientific Pierce 细胞裂解试剂兼容•透明无色,不含不溶性材料•pH 值:4.7±0.2•了解更多信息 ...
Collected cells were washed with ice-cold PBS and lysed in IP-150 lysis buffer (20 mM Tris-HCl pH7.5, 150 mM NaCl, 1 mM EDTA (pH8.0), and 0.5% NP-40) supplemented with protease and phosphase inhibitor cocktails. After incubating for 20 min, samples were spun at 13 000 r.p.m. ...
Tris-HCl Buffer,1.0M,pH7.5500mL Tris-HCl Buffer,1.0M,pH7.5 常温保存 8-iso Prostaglandin F1β (10 mg)9。beta。,11。alpha。,15S-trihydroxy-(8。beta。)-prost-13E-en-1-oic acid; 8-epi-9β-PGF1α|8-iso PGF1β; 8-iso Prostaglandin F1β ...
0.1 mg synthetic EBP50 phospho- peptide (RAHQKR(pS)(pS)KRAPQM) was incubated with 0.1 unit of purified PP1 in PP1 reaction buffer (50 mM Tris-HCl, pH 7.0, 0.1 mM EDTA, 5 mM DTT, 0.01% TX-100 and 1 mM MnCl2)42 or with 0.1 unit of purified PP2A in PP2A reaction buffer (20...
Tris-HCl Buffer,2.0M,pH8.0500mL Tris-HCl Buffer,2.0M,pH8.0 常温保存 高效E.coli感受态细胞制备试剂盒20次 High Efficiency E.coli Competent Cell Preparation Kit 低温 植物组织培养基 RVS肉汤 RVS Broth 250g 抗生素检定培养基3号(usp) 250g
The mixture was stirred at 4°C for 12 h, and the free Dox was removed using an Amicon Ultra-0.5 device (YM10) by adding 500 μL of 50 mM Tris buffer (pH 8.0) for each 100 μL of residual. The solution was also filtered through a 0.45-μm filter to remove Expression and ...
Thermo Scientific Lysozyme is an enzyme characterized by the ability to break down the bacterial cell wall to improveprotein深入閱讀 Have Questions? Quantity: 0.5 mL 產品號碼90082 價格(TWD)/ Each 價格3,120.00 線上優惠2,190.00僅限網絡訂單。
单位定义:一个 单位的GELase 定义为45°C 条件下,GELase Buffer中1小时内制备600mg溶解在1% LMP琼脂糖凝胶DNA所需要的酶量。 储存Buffer: 50% glycerol含有50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 0.1 M NaCl, 0.1% Triton X-100, and 1 mM DTT. 质量控制:每批GELase制备液中未检测到 DNA核酸外切...
A 2 × ligation buffer for linear form DNA [132 mM Tris·HCl (pH 7.6), 13.2 mM MgCl2, 20 mM dithiothreitol, 0.2 mM ATP, 500 mM NaCl, 20% (w/v) polyethylene glycol 6000 (Wako Pure Chemical)] was prepared and used. The NaCl concentration in this buffer was higher...
awas homogenized in buffer containing 20 mM Tris–HCl (pH 正在翻译,请等待...[translate] aRMB250是到广州的费用,预计三天。我可以安排在下周一寄出样品,请确认 RMB250 is to Guangzhou's expense, estimated three days, please confirm.[translate] ...