The over-all 5-methylcytosine (m5C) content of DNA from normal tissues varies considerably in a tissue-specific manner. By high-performance liquid chromatography, we have examined the m5C contents of enzymatic digests of DNA from 103 human tumors including benign, primary malignant and secondary ...
21,22. Subsequently, many known or novel m5C sites have been validated by using advanced high-throughput techniques combined with next-generation sequencing (NGS). These include RNA bisulfite sequencing (RNA-BisSeq)22,23, m5C-RNA immunoprecipitation (RIP)...
threshold was set to 5% (FP% = 0). The original numbers for each site in two replicates (n = 2) are presented together.d, Comparison of the FP rate at non-m5C-modified C sites on ribosomal RNA (n = 1,690) among Zymo EZ RNA Methylation Kit, the three previously repo...
68301-1-Ig targets 5-methylcytosine in IHC, IF, Dot Blot, ELISA applications and shows reactivity with 5mc, chemical compound, m5c samples. Tested Applications IHC, Dot Blot, ELISA Application Description Cited Applications IF Tested Reactivity 5mc, chemical compound, m5c Cited Reactivity human,...
M5C 5-methylcytosine MDA malondialdehyde MeRIP methylated RNA immunoprecipitation NFE2L2 encodes the protein NRF2 NRF2 nuclear factor erythroid 2-related factor 2 NSCLC non-small cell lung carcinoma NSUN2 NOP2/Sun RNA methyltransferase 2 RIP RNA immunoprecipitation ROS reactive oxygen species RT-qPCR re...
(NSUN2 and NSUN6) have been characterized as writers that catalyze m5C deposition on mRNA [15,16]. Aly/REF export factor (ALYREF) [16] and Y-box binding protein 1 (YBX1) [13,17]have been identified as readers recognizing m5C-modified mRNA. Dynamic and reversible m5C modification ...
m5C regulatorBiological processHuman diseaseRNA methylation modifications influence gene expression, and disruptions of these processes are often associated with various human diseases. The common RNA methylation modification 5-methylcytosine (m5C), which is dynamically regulated by writers, erasers, and ...
m5C-marked RNA transcripts (readers) are Aly/REF export factor (ALYREF), Y-box binding protein (YBX)1, YBX2, YTH domain-containing family protein 2 (YTHDF2), radiation sensitive 52 (RAD52), and fragile X mental retardation protein (FMRP).9,11,12,13,14,15,16 mRNA m5C modifications ...
Although 5-methylcytosine (m5C) is a widespread modification in RNAs, its regulation and biological role in pathological conditions (such as cancer) remain unknown. Here, we provide the single-nucleotide resolution landscape of messenger RNA m5C modifica
1Altmetric Metrics Abstract Accurate and systematic transcriptome-wide detection of 5-methylcytosine (m5C) has proved challenging, and there are conflicting views about the prevalence of this modification in mRNAs. Here we report an experimental and computational framework that robustly identified mRNA m5C...