Our data provide evidence that in the chosen cell lines and in so far limited number of patients-derived cells (N=4) the gene expression of PU.1 and its direct targets Cebpa and Cbfb is stimulated by 5-AZA and this effect is further enhanced by G-CSF. Furthermore, marks of activated...
As a mechanistically unbiased starting point to identify targets that modulate the antileukemic activity of 5-Aza, we performed replicate RNA-interference (RNAi) sensitizer screens of 861 kinase, cell cycle, apoptosis and other cancer-associated genes in myeloid cell lines in combination with 5-Aza ...
Deciphering the molecular mechanisms underlying the phenotypic effects of the treatment with demethylating drugs is a crucial step in understanding what genes may be interesting targets for chemotherapy. The available data on the mechanism of action of these drugs strengthen the idea that it is differ...
Collectively, these findings show that aerosolised 5-azacytidine targets the lung, effectively reprogrammes the epigenome of tumours, and is a promising approach to combine with other drugs for treating lung cancer. Similar content being viewed by others Inhalation exposure to cigarette smoke and infl...
stem cell maintenance and drug resistance19. While the functions of lncRNAs have been explored20and growing evidence suggests that they should be considered as drug targets in human diseases21, the mechanisms of regulation of lncRNA expression are much less understood17. In helminths other thanS. ...
Total and nuclear SREBP-2 protein levels (top panel) and relative mRNA expression of its downstream targets (bottom panels) were analyzed by Western blotting and QPCR, respectively. P, SREBP-2 precursor. N, SREBP-2 nuclear form. C, in condition 1, HepG2 cells were incubated without (−...
Collectively, these findings show that aerosolised 5-azacytidine targets the lung, effectively reprogrammes the epigenome of tumours, and is a promising approach to combine with other drugs for treating lung cancer.doi:10.1038/bjc.2013.575Reed, M D...
The chemical inhibitor identified by Bhuvanagiri etal perturbs Nonsense-Mediated RNA Decay (NMD), a RNA surveillance pathway that targets mRNAs harboring premature termination codons (PTCs) for degradation (Kervestin & Jacobsondoi:10.15252/emmm.201404569...
Selected targets were verified on the MDS/CMML cohort. The study presented here showed a stable variant allele frequency and stable global methylation levels in responding patients. A significant demethylation of EZH2 and NOTCH1 was revealed in patients with Aza response. A response to Aza is not...
Epigenetic priming targets tumor heterogeneity to shift transcriptomic phenotype of pancreatic ductal adenocarcinoma towards a Vitamin D susceptible state Article Open access 26 January 2024 KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regul...