BCA法测定蛋白浓度,蛋白样品经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳,转至PVDF膜,5% BSA封闭1.5 h,分别加入GPR43、TPH1、SERT和5-HT3R抗体,4℃孵育过夜,TBST洗膜3次,每次15 min,加入二抗,室温孵育2 h,TBST洗膜3次,每次15 min,加...
[0068] 步骤十一:转膜结束后,加入5%脱脂奶粉(5%milk/tbst)封闭pvdf膜2h; [0069] 步骤十二:抗体孵育:配置5%bsa/tbst,按1:1000比例将兔抗人p-ikbα抗体、兔抗人ikbα抗体、兔抗人p-p65抗体、兔抗人p65抗体、兔抗人p-p38抗体、兔抗人p38抗体、兔抗人p-erk1/2抗体、兔抗人erk1/2抗体稀释于5%bsa中;并置...
图6:使用Phospho-Akt (Thr308) (D25E6) XP® Rabbit mAb #13038,检测经PDGF处理的3T3细胞样本,BSA封闭的结果中可见背景和杂带,而牛奶封闭的结果背景更干净,信号强且清晰。 封闭时使用5%脱脂奶粉-TBST,摇床上室温封闭1h。如后续...
Application Created with Sketch. 图:1/5 TAMRA Antibody (MA1-041) in WB Western blot analysis was performed by loading various amounts of purified TAMRA-BSA protein onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at ...
转膜结束后,迅速取出PVDF膜,放到5%BSA中,室温封闭化;G.取出膜,在摇 床上用TBST洗膜3次,每次5min化赔育袋中加入用封闭液稀释的VEGF-A度osterl:200) 和GAPDH度oster1:2000),4 °C赔育过夜;I.TBST洗膜3次,每次5min,辣根过氧化物酶 (HR巧标记的羊抗兔二抗(Jacksonl:2000)和辣根过氧化物酶(HR巧标记的...
FH Kamal (Verified Customer) (01-26-2024) Empty heading Works well when diluted in 1X TBST. Applications:Western Blot Primary Antibody Dilution:1:2000 Cell Tissue Type:Mouse liver tissue
TBST冲洗,5分钟后再次冲洗放5分钟。后加3%BSA封闭1小时。配制一抗,加一抗4度过夜。第二天,拿出标本室温下放置20分钟。TBST洗涤3次,15分钟每次。加入二抗,室温放置1小时。TBST洗三次,每次15分钟。DAB显色,注意把握时间。复染细胞核(Hemotoxylin)约5-8秒。龙头水冲洗一次。醋酸(acetic acid)5秒,后氨水(...
(#9172 Cell Signaling technology), anti-phospho-Y701-STAT1 (#9167 Cell Signaling technology), anti-SOCS-5 (#sc-5607, Santa cruz biotechnol- ogy) anti-JEV NS1 (#ab41651; Abcam) and anti-β- tubulin (#ab6046; Abcam) primary antibodies were used after diluting in 5% BSA in TBST ...
公司成立:21年 注册资本:1000万元 企业类型:有限责任公司 主营产品:tris,甘氨酸,琼脂粉,潮霉素B,SDS,BSA 公司地址:联东U谷景盛南四街85A三层 进入店铺 询盘 店内推荐 10×TBE缓冲液 ￥140.00 5×TBE 缓冲液 ￥120.00 1×TBST缓冲液 ￥60.00 5...
(Product ## 62288), and blocked with 5% BSA in TBST for at least 1 hour at room temperature. IgA heavy chain was detected at ~55kD using a mouse anti-human IgA monoclonal antibody (Product ## MA5-11208) diluted to a concentration of 0.5ug/ml in blocking buffer, ...