5α-reductase deficiency results in androgen insensitivity due to abnormal 5α-R2. Affected patients are XY individuals with a very peculiar form of male pseudohermaphroditism: they have feminine genitalia at birth and masculinize at puberty. Different mutations, spannning the whole coding portion ...
However, when incubations were performed in the presence of varying amounts of NADPH, it turned out that RA exerts its effect by competitive inhibition of the cofactor action. Although the severe toxicity of RA precludes its systemic use as a 5α-reductase inhibitory drug in humans, the ...
The apparent dissociation constant for NADPH cofactor is similar for both enzymes. DHT is necessary for normal growth and function [4], development of benign prostatic hyperplasia (BPH) [5] and probably also for initiation and maintenance of prostate cancer (PCa) [6]. The prostate is not a ...
5 alpha-Reductase activity was found only in particulate subcellular fractions of a homogenate of epidermal keratinocytes when assayed with tritium-labeled progesterone as the substrate and NADPH as the cofactor. In addition to 5 alpha-reductase activity, other enzymatic activities found in epidermal ...
Anti-CYB5R2抗体CYB5R2 (cytochrome b5 reductase 2), also known as B5R.2, is a 276 amino acid protein that contains one FAD-binding FR-type domain and belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Existing as two alternatively spliced isoforms, CYB5R2 reduces 2-...
1. Folate, an essential micronutrient, is a critical cofactor in one-carbon metabolism. Mammalscannot synthesize folate and depend on supplementation to maintain normal levels. Lowfolate status may be caused by low dietary intake, poor absorption ...
Further, the identity of amino acid 117 determines whether an AKR can function as a 5β-reductase by reorienting the substrate relative to the nicotinamide cofactor. This study provides functional evidence that utilization of modified catalytic residues on an identical protein scaffold is important for...
Flavin adenine dinucleotide (FAD) is a cofactor for cytochrome-b5 reductase, the enzyme that maintains hemoglobin in its functional reduced state, and for glutathione reductase. This enzyme also protects erythrocytes from oxidative damage. FAD is a redox cofactor of several essential reactions in metab...
Steroid reductases 5α (SRD5As) catalyse the conversion of T to DHT in the classic androgen production pathway, or from 17-hydroxyprogesterone to 17OH-dihydroprogesterone, and androstenedione to androstanedione in alternate pathways leading to DHT. There are two enzymes with differential expression,...
Biological ActivityAnticancer agent. Metabolized to form fluorodeoxyuridine monophosphate (FdUMP), fluorodeoxyuridine triphosphate (FdUTP) and fluorouridine (FUTP). FdUMP inhibits thymidylate reductase causing a reduction in dTMP synthesis. FUTP and FdUTP are misincorporated into RNA and DNA respectively...