Human PPP4C qPCR Primer Pair,即人PPP4C qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR),是一...
这表明EBV与宿主基因组的相互作用在宿主基因组的B室中诱导增强子活化,并形成异常染色质环以上调靶基因。 通过RT-qPCR证实,PLA2G4A在C666-1 NPC细胞中的表达显著高于正常NP69T细胞(图7b)。公共RNA-seq数据显示,在两个独立的队列中,临床NPC样本中PLA2G4A的表达显著高于正常样本(图7c)。然而,临床NPC样本中PLA2G4A...
方法: 1.TIMER2.0网站和TCGA数据库分析Sema4C在肝癌患者肿瘤组织和正常组织之间的表达差异,用UALCAN,Kaplan-Meier,GEPIA2网站对HCC患者中Sema4C的表达与生存周期的相关性进行分析; 2.构建Sema4C过表达Li-7细胞系和Sema4C敲低HepG2细胞系,qPCR和WesternBlot实验检测Sema4C的过表达和敲低作用; 3.CCK8和克隆形成实验...
qPCR,全称为Quantitative Polymerase Chain Reaction,即定量聚合酶链式反应,也被称为实时荧光定量聚合酶链式反应(Real-time Quantitative PCR)。这项技术是一种用于测量特定DNA或RNA序列数量的分子生物学方法,它在PCR技术的基础上进行了改进,通过实时监测DNA或RNA的扩增过程,实现了对目标基因的定量分析。 以下是qPCR技术的...
All the CNVs were validated using qPCR (Supplementary Fig. S1). The sizes of the CNVs were 22−198 kb. Two deletions (in cases #9 and #12) overlapped with the exons encoding the JmjC domain, which plays a central role in histone lysine demethylation. In case #9, the mRNA was ...
(ANOVA) for random measures was employed in experiments comparing multiple groups or multiple time points and post hoc Bonferroni’s or Tukey’s test for multiple comparisons was performed, as indicated. In qPCR analyses, ANOVA with Holm–Sidak correction for multiple comparisons was employed. For ...
5.实时荧光定量逆转录PCR(Real-time RT-PCR, RT-qPCR) Real-time RT-PCR是qPCR和RT-PCR的组合,其中的“RT”是Reverse transcription(逆转录)的意思,所以RT-qPCR就是结合了荧光定量技术的逆转录PCR,即以mRNA或总RNA为模板,先反转录得到cDNA,再以c...
您是不是在qPCR引物设计中感到困惑:如何使引物特异性最佳?有没有什么一劳永逸的方法?在设计qPCR引物时,通常要留意以下几点—— 引物尽量要跨内含子设计 产物长度100-300 bp TM值尽量接近60℃且上下游引物尽量接近 引物末端最好是G或C 第一步:查询基因 ...
一、PCR-ELISA法制备qPCR标准曲线 1. 准备试剂和设备 在进行PCR-ELISA法制备qPCR标准曲线前,需要准备以下试剂和设备: - PCR反应体系:包括模板DNA、引物、酶、缓冲液等。 - PCR产物:需要纯化PCR产物,可以使用PCR纯化试剂盒。 - 固相板:可以使用带有特异探针的固相板。 - 标记引物:可以使用...
Real-Time qPCR mRNA was isolated from both control and PP4cfl/fl;Emx1Cre cerebral cortex using TRIzol reagent (Invitrogen) and cDNA was synthesized from 3 μg of total RNA using Superscript II with random primers (Invitrogen). Real-time PCR was performed on a C1000 Thermal Cycler (Bio-Ra...