产品名称 4×Multiplex PCR Master Mix for Pathogen 产品规格 24 T 用途范围 仅做科研,不用于临床 加工定制 否 商品货号 13592ES 商品规格 24 T 生产厂家 翌圣生物科技(上海)股份有限公司 是否进口 否 规格编码 13592ES24 最小起订量 1.00 发货地 上海 售卖区域 全国 可售卖地 全国 类型 科研...
4×Multiplex PCR Master Mix for Pathogen以热启动多重Taq酶制剂与4×扩增缓冲液为组分配置成预混液。本预混液可快速便捷地用于多重PCR反应,扩增子GC含量25-75%范围内可以有效扩增。具备高均一性、高特异性和高灵敏度的特点,同时严控背景菌。本试剂可兼容30~100对以内不同大小片段的多重扩增,也可用于进行数...
4×Multiplex PCR Master Mix for Pathogen以热启动多重Taq酶制剂与4×扩增缓冲液为组分配置成预混液。本预混液可快速便捷地用于多重PCR反应,扩增子GC含量25-75%范围内可以有效扩增。具备高均一性、高特异性和高灵敏度的特点,同时严控背景菌。本试剂可兼容30~100对以内不同大小片段的多重扩增,也可用于进行数...
using sybr green master mix (yeasen biotechnology, shanghai, china). for mrna quantification, the 2 -ΔΔct method was used. gapdh served as an endogenous control. the primer sequences used for qrt-pcr are shown in the supplementary table. western blotting cellular proteins were extracted ...
For Oxford Nanopore sequencing, the Midnight primer kit was used as described previously1. cDNA synthesis was performed on the extracted RNA using the LunaScript RT mastermix (New England Biolabs), followed by gene-specific multiplex PCR using the Midnight primer pools, which produce 1,200-bp am...
(6–8 weeks old) were obtained from the Model Animal Research Center of Nanjing University (Nanjing, China) and housed in specific pathogen-free conditions at Nanjing First Hospital Animal Center, Nanjing, China, in a room controlled for temperature (21 ± 2 °C), humidity (55 ±...