CHINA Tel(86)-21-58794900.4140 1 RT -PCR Q uick Master Mix Contents CAUTION All reagents in this kit are intended for research purposes. Do not use for diagnosis or clinical purposes. Please observe general laboratory precaution and utilize safety while using this kit. PCR-311 50 reactions ...
Power SYBR Green PCR Master Mix (2X)10 1X Reverse Primer Variable 50 to 300 nM Forward Primer Variable 50 to 300 nM Template Variable 1 to 100 ng Nuclease-free water Variable - c. Mix gently. Do not vortex. Centrifuge briefly and then prepare the PCR reaction plate.2Set up the pla...
Contents & Storage • 2X PCR Master Mix, 4 x 1.25 mL• Nuclease-free water, 4 x 1.25 mLStore at -20°C. 浏览此商品的顾客同时也查看了 DreamTaq Green PCR 预混液 (2X) Catalog number: K1081 241.00 / 200 reactionsAdd to cart SYBR™ Safe DNA 凝胶染料 Catalog number: S33102 1,365...
3. Mix the contents gently and briefly centrifuge. 4. Place the PCR tube in a thermocycler and program the following cycling conditions: Initial denaturation: 95°C for 5 minutes. Denaturation: 95°C for 30 seconds. Annealing: 60°C for 30 seconds. Extension: 72°C for 30 seconds. Final...
®F0925K SYBR ® Green Realtime PCR Master Mix -Plus- QPK-212 1mLx5 Store at -20°C, protected from light Contents [1] Introduction [2] Components [3] Primer design [4] Detection [5] Specimens [6] Protocol 1. Intercalator assay protocol using ABI PRISM ® 77...
Use this enyzme mix stable for 24 hours at +15 to +25°C. • Reduce Setup time. Store master mixes at +2 to +8°C for immediate use for up to one month. Contents 2x concentrated master mix containing FastStart Taq DNA Polymerase, Reaction Buffer, and Nucleotides (dATP, dCTP, dGTP...
PCR Master Mix Reaction Plate Plate Document Conditions and STANDARD Standard Start the Run (OR) Specify Running an Prepare the Prepare the Create an AQ Thermal Cycling Chapter 5 FAST AQ Plate Before You Begin PCR Master Mix Reaction Plate Plate Document Conditions and Troubleshooting FAST Fast ...
Reaction FormatSuperMix or Master Mix Unit Size1000 reactions Contents & Storage • 2X DreamTaq Green PCR Master Mix, 2 x 1.25 mL • Nuclease-free water, 20 x 1.25 mL Store at -20°C. Figures Longer PCR fragments, higher yields with DreamTaq Green DNA Polymerase ...
反应体系为:1.1×T3 Super PCR Mix 21 μL,10 μmol·L-1上游引物1 μL,10 μmol·L-1下游引物1 μL,模板DNA2 μL;bosPDE反应程序为:98℃,2 min,[98℃,10 s,(46.3℃、47.8℃、50.9℃、52.3℃、53.7℃、54.9℃、58.0℃、60.0℃)72℃,1 min]35个循环,72℃,2 min,4℃保存;LcoR反应程序为:...
.roche-applied-scienceFastStartUniversalSYBRGreenMaster(ROX)1.WhatthisProductDoesContentsTheFastStartUniversalSYBRGreenMaster(ROX)isaready-to-use,2×concentratedmastermixthatcontainsallthereagents(exceptprimersandtemplate)neededforrunningreal-timeDNAdetectionassays,includingqPCRand2-stepqRT-PCR,intheSYBRGreenIdetecti...