3T3-L1细胞的培养及成脂诱导是进行代谢性疾病研究的重要手段。通过严格控制培养条件和成脂诱导过程,可以获得高质量的类脂肪细胞,为进一步研究代谢性疾病的发病机制提供有力支持。 3T3-L1细胞培养及成脂诱导方面的参考文献: Zheng J, et al. (2021) PPARγ Regulates Adipogenesis of 3T3-L1 Cells by Modulating Mi...
Isorhamnetin represses adipogenesis in 3T3-L1 cells. Obesity (Silber Spring), 2009, 17(2): 226-232.Lee J, Jung E, Lee J, Kim S, Huh S, Kim Y, Kim Y, Byun SY, Kim YS, Park D. 2009. Isorhamnetin represses adipogenesis in 3T3-L1 cells. Obesity (Silver Spring). 17:226-232....
图2 STC、STCF3和LMPST对PA处理的3T3-L1细胞的细胞内脂质抑制作用 LMPST2对3T3-L1细胞脂肪生成和脂解相关蛋白表达水平的影响 为了澄清脂肪生成和脂解途径的机制,进行了Western blot分析以确定每种蛋白质表达水平。结果表明,在PA处理的3T3-L1细胞中,PPAR-γ和C/EBP-β的蛋白表达水平升高(图3)。然而,LMPST2显著...
Methods: The adipogenesis of 3T3-L1 cells was induced by stimulation with a differentiation cocktail (DMI) medium for 8 days. MTT assay was used to measure the cell viability and Oil Red O staining was used to evaluate the adipogenesis of 3T3-L1 cells. The total level of triglyceride and r...
7‑MEGA™ inhibits adipogenesis of 3T3-L1 adipocytes First, the current study examined the impact of 7‑MEGA™ on the viability of 3T3-L1 preadipocytes. The cells were cultured and treated with 125, 250, and 500nL/mL 7‑MEGA™ for 72 h. Cell viability was determined using the ...
[2]Berberine inhibits SREBP‐1‐related clozapine and risperidone induced adipogenesis in 3T3‐L1 cells[J].YueshanHu,EricKutscher,Gareth E.Davies.Phytother.Res..2010(12) [3]Statin use and cancer risk:a comprehensive review[J].Denise M Boudreau,Onchee Yu,Jeanene Johnson.Expert Opinion on Drug ...
Addition of BSO to 3T3-L1 cells 3 days after the initiation of differentiation impairs triglyceride accumulation and expression of proteins induced in late adipogenesis. Of note, proteins that increased early during adipogenesis are also diminished, suggesting that BSO causes de-differentiation of these...
3T3-L1adipocyte 全称 3T3-L1细胞中文名：小鼠胚胎成纤维细胞 来源 从3T3细胞（Swissalbino小鼠）中经克隆分离得到的连续传代的亚系。形态特性 成纤维细胞样 生长特性 贴壁生长该细胞从快速分裂到汇合和接触性抑制状态经历了前脂肪细胞到脂肪样细胞的转变。3T3-L1前脂肪细胞的鉴定1形态学鉴定3T3一L1前脂肪细胞可以通过...
Adipogenesis and fat-cell function in obesity and diabetes. Trends in Molecular Medicine, 8(9), ...
Skimmed milk fermented by lactic acid bacteria inhibits adipogenesis in 3T3-L1 pre-adipocytes by downregulating PPARγ via TNF-α induction in vitro。 https://doi.org/10.1039/d1fo00076d 专家简介 Seok-Seong Kang首尔 东...