Kramer AH, Edkins AL, Hoppe HC, Prinsloo E. Dynamic Mitochondrial Localisation of STAT3 in the Cellular Adipogenesis Model 3T3-L1. J Cell Bio- chem 2015; 116:1232-40; PMID:25565605; http:// dx.doi.org/10.1002/jcb.25076A. H. Kramer, A. L. Edkins, H. C. Hoppe, E. Prinsloo, ...
To date, many studies have focused on the inhibition of adipogenesis. However, the notable ability of adipocytes to resist apoptosis is poorly understood [18]. Cell death, including apoptosis and necrosis, are followed by the cleavage of proteins and DNA [19]. These two pathways of cell death...
The 3T3-L1 murine preadipocyte cell line is a commonly used tool for analysis of the subcellular pathways involved in preadipocytic cell differentiation (a process also commonly known as adipogenesis). The major characteristic of adipogenesis is the intracellular accumulation of membrane-bound lipid dr...
3T3-L1 cells are widely applicable for the study of adipocyte biology. Over the last decade, the 3T3-L1in vitrocell model has been widely used to study molecular and cellular processes ofadipogenesis. Studies using this cell line have focused on evaluating the role, function and possible mechani...
In conclusion, the essential oil of Alpinia galanga can inhibit the adipogenesis pathway in 3T3-L1 preadipocytes and its freeze-dried powder potentially reduces the body weight, body fat ratio and severity of fatty liver in obese BALB/c mice by retarding the accumulation of fat.肥胖是由於熱攝取...
with one or more ribosomes attached, within the first six hours of adipogenesis in 3T3-L1 cells, a widely used model for the study of adipogenesis and function of adipocytes [6–8]. Adipogenesis is a complex process, and a number of factors are important for its regulation. Many genes ...
We found that echinomycin, a potent HIF-1 inhibitor, completely inhibited adipogenesis in 3T3-L1 WAT preadipocytes by affecting the early phase of mitotic clonal expansion. The dose required to exert the effect was surprisingly low and the time was short. Interestingly, its inhibitory effect was ...
The transcription factors PPARγ, LXRα, and SREBP-1 are induced during adipogenesis, and their transcriptional activities may be regulated by PUFAs. Previously, we have shown that the PPARγ agonist darglitazone reduced resistin mRNA in the presence of serum (18). In the present study, we ...
Therefore, in this study, to elucidate the effects of TGF-β isoforms (TGF-β-1, -2, and -3) on adipogenesis, lipid production and gene expression of adipogenesis-related molecules of 2D and 3D cultures of 3T3-L1 cells were evaluated. Materials and methods Adipocyte cultures and the differe...
Curcumin inhibits STAT3 activity on adipogenesis induction in 2D and 3D cell cultures; however, the inhibition is less in the 3D culture. Culturing 3T3-L1 preadipocytes in 3D cell cultures maintained a high STAT3 activity and C/EBPα expression levels compared with 2D systems. Curcumin’s cellula...