Transgene Organism Tissue Morphology Culture Properties mCherry-p65 Mus musculus Embryo Fibroblast Adherent mCherry-p65 NIH/3T3 Cells,即mCherry-p65 NIH/3T3细胞,是一种可以通过CMV启动子组成型表达mCherry-p65融合蛋 白的多克隆NIH/3T3稳定细胞株(Polyclonal NIH/3T3 stable cell line),主要适合用于通过...
组织来源Tissue and Cell Type 胚胎 形态Morphology 成纤维细胞 培养基和添加剂Complete Growth Medium and Culture Conditions DMEM培养基(添加NaHCO3 1.5g/L),90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。 供应限制Permits and Restrictions A。仅供研究之用。 REFERENCE 886: Green H...
Data is presented that demonstrates changes in cell shape with increasing concentrations of cinnamon extract. Evidence of lipid accumulation is visualized by oil red O staining as early as 0.5 weeks following cinnamon treatment. Monolayer cells exhibit round morphology when treated with cytochalasin D, ...
Cell Type: Embryonic mouse fibroblast preadipose cell line Accession ID: L1, CVCL_0123; F442A, CVCL_0122 Organism: Mouse Morphology: Fibroblastic, can be differentiated to adipocytes Source: Mouse embryo Biosafety Level: BL1 Growth Conditions: Cells grow in 10% CO2 at 37C in a humi...
本细胞株详细信息如下: General Information Cell Line Name p53-TA-Luc-EF1α-mCherry NIH/3T3 Cells Transgene p53-TA-Luc-EF1α-mCherry-T2A-Puro Synonyms / Organism Mus musculus (Mouse) Tissue Embryo Cell Type Stable Cell Line Morphology Fibroblast Disease - Strain NIH/Swiss Biosafety Level*...
As one of the most commonly utilized cell lines, the NIH3T3 cell line has been incorporated in studies for a range of mechanistic and cell based assays, including protein functional analysis. The morphology of the cells are adherent, fibroblastic and are considered to be among the relatively ea...
组织来源 Tissue and Cell Type 胚胎 形态/Morphology 成纤维细胞样,贴壁生长 培养基和添加剂/Complete Growth Medium and Culture Conditions: 90%DMEM(MD0100)+10%小牛血清(SD0400)+G/A (SD0038) DMEM完全培养基(MD0101) 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。 冻存条件:基础培养基+5%DMSO+20%FBS...
Tissue and Cell Type胚胎 形态 Morphology成纤维细胞,贴壁生长 培养基和添加剂 Complete Growth Medium and Culture ConditionsNIH/3T3 完全培养液 配方(100 ml) DMEM (Invitrogen, 11960-044)88 ml 新生牛血清 (Gibco)灭活后使用 10 ml Glutamax (Invitrogen, 35050061) 1 ml ...
Cell Type: Wild-type murine MET and murine MET mutations (based on human nomenclature) V1206L, V1110I, H1112Y, H1112L, H1124D, M1149T, L1213V, V1238I, Y1248H, Y1248D, M1268T in NIH3T3 cells Accession ID: P08581 Organism: Mouse Morphology: Fibroblast Source: Embryo/establi...
一定要在细胞状态好的情况下诱导分化,3T3-L1长得非常快,如果长满以后不换液,会有大批细胞死亡,判断细胞好坏的标准是cell confluent后,培养基中很少有漂浮的死亡细胞。所以诱导前传代的细胞在接种时数量不要太大,最好在10*4数量级,这样让细胞长3天后铺满,再换液,再让细胞长2-3天,这时的细胞会退出生长周期,进入...