Therefore, in this study, to elucidate the effects of TGF-β isoforms (TGF-β-1, -2, and -3) on adipogenesis, lipid production and gene expression of adipogenesis-related molecules of 2D and 3D cultures of 3T3-L1 cells were evaluated. Materials and methods Adipocyte cultures and the differe...
To investigate the effect of GE on 3T3-L1 cell differentiation and corresponding lipid acquisition, cells were differentiated at one half the LC50 (20.2 μg ml−1) and the LC50 (40.3 μg ml−1) concentrations of the undifferentiated 3T3-L1 cells with untreated cells acting as ...
Sinapic acid was added to the 3T3-L1 cells on d0 and d2. For p38 MAPK inhibition or CREB inhibition, 3T3-L1 adipocytes were incubated with 10 μM of SB203580 (Sigma) or 10 μM of 666-15 (Tocris Bioscience, Bristol, UK) for 24 h, respectively. After the treatment, the ...
The purpose of this study was to use specific silencing of TNSALP mRNA , using short interfering (si) RNA , to investigate the role of TNSALP in intracellular lipid accumulation in 3T3‐L1 and HepG2 cells. Cellular activity of TNSALP was measured using an automated colorimetric assay, and ...
Protein extracts prepared from 3T3-L1 cells expressing either the WT or the mutant FTO proteins were used in DIGE experiments. Analysis of the data revealed the number of spots matched to every member and there were 350 ± 20 spots with 30.5% overall mean coefficient of variation. Eleven ...