generationVSV.Gpseudotypedlentiviralpackagingprotocol Seed2.5x10^6lowpassage(lessthanP20)293Tcellsper15cmdishin15mlDMEM with10%serumand1%Pen/Strep.Forastandardprepof12dishesyouwillneedtostart with3-4dishes.Growuntil90%confluentandthensplit1:3–1:4togivetwelve15cm dishes.Allowcellstogrownuntil70%confluen...