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these diseases.; To investigate the intracellular distribution of 3R tau and 4R tau isoforms in neurofibrillary pathology in AD, we generated and characterized a 4R tau-specific monoclonal antibody that was then used in conjunction with a previously characterized 3R tau-specific monoclonal antibody....
Subsequently, we performed an immunohistochemical labeling of the 3R tau isoform on serial sections of the adult rat brain. Interestingly, the 3R tau isoform is not only expressed in neuronal precursor cells. It is also present in mature neurons of the olfactory bulb, magnocellular neurosecr...
In situ proximity ligand assays (PLA) With PLA, primary antibodies raised in different species are used to detect different proteins. Secondary antibodies specific to each primary antibody that include a short sequence of DNA are used to detect the presence of the primary antibodies. If both prima...
Gli-3Gli-3R及胶质瘤恶性程序及增殖指数的关系.pdf,中文摘要 目的 1.研究Gli.3在人胶质瘤病理标本中的表达情况。 2.探讨Gli.3/Gli.3R与胶质瘤WHO分级及增殖指数的关系。 方法 1.采集手术切除、经组织病理检查证实为胶质瘤的病理标本。 2.利用免疫组化的方法检测Gli
After several washes, membranes were incubated for 2 h with an appropriate secondary antibody conjugated with horseradish peroxidase (1:5000, Vector). The membrane was visualized using a chemiluminescent mix of 1:1 (0.5 M luminol, 79.2 mM p-coumaric acid, 1 M Tris-HCl, pH 8.5) and (8.8 ...
We blocked the membranes with 5% BSA in PBS plus 0.1% Tween-20 for 1 h at room temperature and then incubated at 4 ◦C overnight with primary antibody: mouse anti-β-actin (1:10000; Sigma- Aldrich), mouse anti-GRP78 (1:500, Sigma-Aldrich), mouse anti-CVβ (1:1000, Invitrogen)...