3D 共聚焦显微镜 CLSM.pdf,3D 共聚焦显微镜CLSM 1. 仪器功能: 共聚焦显微镜最大的优点就是可以只检测一个聚焦平面的信号。样品聚焦平面和 检测器(光电倍增管)之前均有一个针孔,针孔的设置可以有效地滤除非聚焦平面的信 号,增加显微镜的信噪比。激光扫描显微镜能够逐点和
The z-stack images were obtained using Zeiss-410 CLSM system with the stepsize of 0.4 um in air along the z-axis. Ideally, a sphere should be shown as a disk with diameter d in each image of the stack. But the measured data showed significant deviation in d vs z relation from the ...
CLSM images of the surface of the developed food model systems: (a) 0% fat, (b) 10% fat, (c) 20% fat, (d) 40% fat and (e) 60% fat. The samples were stained in red with 0.01% Rhodamine B (protein phase) and in green with 0.1% BodiPy (fat phase). All z-stack images we...
4-C). Confocal laser scanning microscope (CLSM) image slices also validated the co-localization (data not shown). Download: Download high-res image (442KB) Download: Download full-size image Fig. 4. Confocal microscopy images of A549 alveolar epithelial cells uptake of ATRA-PLGA NPs treatment...
Blending law can follow the phase behaviour of agarose-gelatin composite gels.3D CLSM imaging paired with image analysis generates a Z-stack of the composite.Volumetric quantification of agarose-gelatin phase volumes matched the predictions of blending law.A rapid microscopy protocol can replace blending...
5D) of the strand at one selected position of the cLSM image stack proved the attachment to the strand surface in contrast to the homogeneous distribution throughout the entire thickness of the algMC strand as observed at early time points (Fig. 5A,C, day 7). Figure 5 Cytoskeletal ...
(b and c), a 3D rendering was edited by Adobe Photoshop CC software preserving the original parameters of image acquisition (d) Fluorescence image showing IFN-DCs (green) crossing connecting-channels facing RI space upon phagocytosis of PKH26-stained SW620 cells (red) as evaluated by CLSM ...
Myoanatomy of the mouth cone and introvert ofEchinoderes spinifurca.(A-D)3D reconstructions of confocal z-stacks in the head region.(E-L)confocal z-stack projections of phalloidin staining in the head region.(A, C)apical view, ventral side is down.(B, D)ventral view, anterior to the...
Each red image presents the maximum intensity projection of deconvolved stack of CLSM images. Bar = 2 μm. Images are representative for at least 20 independent experiments. Grey images represent 3D models of intact pea (C, E) and bean chloroplasts (D, F) created after deconvolution. ...
European Laboratory for Non-linear Spectroscopy CLSM: Confocal Light Sheet Microscopy ROI: Region Of Interest PC: Phase Correlation NCC: Normalized Cross-Correlation I/O: Input/Output V: vertical H: horizontal D: depth GB: GigaByte TB: TeraByte. ...