(2)多维图像拍摄: a:Z 轴扫描及三维构建(Z-Stack):LSCM 通过 Z-stack 可以对样品进行“光学切片”, 用于分析样品的三维立体结构,目标分子的空间分布等。 b:时间序列扫描(Time Series):时间序列扫描主要用于获取反映样品连续变化的图像信息。时间序列扫描(需配备活细胞工作站)可实现对活细胞的实时动态观测,记录细胞...
Because of these optical advantages, the confocal laser scanning microscope (CLSM) can clearly image thin optical sections from within thick fluorescence-labeled living specimens. A stack of optical sections is easily combined to reveal three-dimensional (3D) fluorescent marker distributions with ...
Confocal Laser Scanning Microscopy (CLSM) and 3D reconstruction Confocal imaging was performed with an LSM 510 (Carl Zeiss Inc.), an LSM700 mounted on an Axio Imager up- right microscope (Carl Zeiss Inc.), or a Leica DM 5000 CS with SP5 laser scanning unit. Confocal z-stack pro- ...
a:Z 轴扫描及三维构建(Z-Stack):LSCM 通过 Z-stack 可以对样品进行“光学切片”, 用于分析样品的三维立体结构,目标分子的空间分布等。 b:时间序列扫描(Time Series):时间序列扫描主要用于获取反映样品连续变化的图像信息。时间序列扫描(需配备活细胞工作站)可实现对活细胞的实时动态观测,记录细胞内特定成分的变化并...