Tissue microarray analysis of human breast cancers was used to measure PDK1 expression in invasive tumors by IHC./p pResults/p pEnhanced invasion on Matrigel in PDK1-expressing cells was accompanied by increased MMP-2 activity resulting from stabilization against proteasomal degradation. Increased MMP-...
Western blot analysis with an anti-PDK1 antibody revealed that KF58333-mediated PDK1 degradation was partially blocked by proteasome inhibitors and not by other protease inhibitors (Fig.6 A). Quantification of the soluble PDK1 amount revealed that proteasome inhibitors increased the level of soluble ...
For example, GSK-3β can enter the nucleus where it phosphorylates the cell cycle protein cyclin D1, thus targeting it for rapid degradation (33). In addition, phosphorylation of translation initiation factor eIF2B by GSK-3β inhibits protein translation, leading to initiation of apoptosis an...
Mitotic and stress-induced phosphorylation of HsPI3K-C2alpha targets the protein for degradation. Activation of the phosphoinositide 3-kinases (PI 3-kinases) has been implicated in multiple cellular responses such as proliferation and survival, membrane... SA Didichenko,CM Fragoso,M Thelen - 《Journ...
Activation of cardiac phosphoinositide 3-kinase α (PI3Kα) by growth factors, such as insulin, or activation of PI3Kγ downstream of heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors stimulates the activity of the kinase Akt, which phosphorylates and inhibits glycogen synt...
Biochemical analyses indicated that PI3K–Akt signaling increased nuclear total β-catenin and P-β-catenin 552 levels and reduced N-terminal β-catenin phosphorylation, which is associated with degradation. PI3K inhibition in interleukin-10 / mice impaired colitis-induced epithelial Akt and β-catenin...
Proteasomal degradation of the FoxO1 transcriptional regulator in cells transformed by the P3k and Akt oncoproteins The P3k oncoprotein [homolog of the catalytic subunit p110伪 of class 1A phosphoinositide 3-kinase (PI3K)] and its downstream effector Akt induce oncogenic... M Aoki,H Jiang,PK Vog...
Activation of class Ia PI3K (phosphoinositide 3-kinase) produces PtdInsP3, a vital intracellular mediator whose degradation generates additional lipid sign... IH Batty,JVD Kaay,A Gray,... - 《Biochemical Journal》 被引量: 48发表: 2007年 Human erythrocyte membranes exhibit a cooperative calmodulin-...
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration dat...
The proteasome is a multiprotein complex that is involvedin the intracellular protein degradation in eukaryotes. Here, we show thathuman malignant glioma c... B Wagenknecht,M Hermisson,P Groscurth,... - 《Journal of Neurochemistry》 被引量: 66发表: 2010年 加载更多来源...