The C-terminal part of the enzyme harbors enoyl-CoA hydratase activity and is able to converttrans-crotonyl-CoA to 3-hydroxybutyryl-CoA. The N-terminal part of FadB' comprises an NAD+binding site and is responsible for 3-hydroxyacyl-CoA dehydrogenase activity converting (S)-3-hydroxybutyryl-...
The activity of the long-chain 3-hydroxyacyl-CoA dehydrogenase and long-chain 3-oxoacyl-CoA thiolase in fibroblasts from the patient's parents was ... S Jackson,RS Kler,K Bartlett,... - 《Journal of Clinical Investigation》 被引量: 435发表: 1992年 Long-chain 3-hydroxyacyl-CoA dehydrogenase...
Table 2. Fatty acid oxidation, acyl-CoA dehydrogenase, 3-ketoacyl-CoA thiolase, and 3-hydroxyacyl-CoA dehydrogenase activity measurements in cultured skin fibroblasts o fuatient and control subjects Activity measured Substrate Patient Controls (n = 5)* Fatty acid oxidation? [ 1-14C]-succ~nic [...
Summary Peroxisomal β-oxidation proceeds from enoyl-CoA through d-3-hydroxyacyl-CoA to 3-ketoacyl-CoA by the d-3-hydroxyacyl-CoA dehydratase/d-3-hydroxy-acyl-CoA dehydrogenase bifunctional protein (d-bifunctional protein), and the oxidation of bile-acid precursors also has been suggested as bein...
Both wild type domains were enzymatically active, with the B domain showing the highest activity with short chain and the A domain with medium and long chain (3R)-hydroxyacyl-CoA substrates. The data show that the dehydrogenase domains of yeast MFE-2 have different substrate specificities ...
3-Hydroxyacyl-CoA dehydrogenase (LCHAD) (EC 1.1.1.35), the third enzyme of the mitochondrial fatty acid oxidation, catalyses the reversible dehydrogenation of 3- hydroxyacyl-CoA into 3-ketoacyl-CoA. This enzyme was only recently found to be a part of the multienzyme complex also harbouring ...
(1979) Activities of malate dehydrogenase, 3-hydroxyacyl-CoA-dehydrogenase and fructose-1,6-diphosphatase with regard to metabolic subpopulations of fast- and slow-twitch fibers in rabbit muscles. Histochemistry 60: pp. 9-19Spamer C, Pette D (1979) Activities of malate dehydrogenase, 3-...
It proved that measuring the activity of short-chain 3-hydroxyacyl-CoA dehydrogenase (SCHAD) could reveal that the body had been frozen.doi:10.1016/S0379-0738(01)00566-7A. MirasH. Yapo-EtteC. Vianey-SabanD. MalicierL. FantonElsevier Ireland LtdForensic Science International...
(1980a) A bifunctional enzyme from glyoxysomes: Purification of a protein possessing enoyl-CoA hydratase and 3-hydroxyacyl-CoA dehydrogenase activity. Eur. J. Biochem. 107 , 79–86Frevert, J., and Kindl, H. (1980) Eur. J. Biochem. 107, 79–86 View Article...
Limited proteolysis of the bifunctional enzyme with 卤-chymotrypsin yielded a peptide mixture containing a 36 kDa fragment, the mixture showing about 76% of the original enoyl-CoA hydratase activity but no 3-hydroxyacyl-CoA dehydrogenase activity. Comparison of the peptide maps of the purified enoyl...