This chapter describes protocols for two-dimensional (2D) gel electrophoresis (isoelectric focusing [IEF] followed by sodium-dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis [PAGE]), stainingKim H, Eliuk S, Deshane J, Meleth S, Sanderson T, Pinner A, et al. 2D gel proteomics: An ...
例如: “The protocol of Sambrook et al. (1989) was adapted in the following way.” “用以下方式调整了Sambrook等人(1989)的方案。” 这意味着对该方案进行了修改,并且将在随后进行描述。 adopt某事/物即录用、接受或遵循计划。例如: “The ...
Two-dimensional (2D) difference gel electrophoresis (DIGE) is a technique for comparative proteomics, which improves the reproducibility and reliability of differential protein expression analysis between samples. The standard sample included in every gel (Cy2) comprises equal amounts of each sample to...
Reverse primer: 5’- TGGGTCATCTTCTCGCGGTTGG-3’ Cycling conditions are listed in Table 1. The PCR products were detected using GelRedTM (Biotium) following agarose gel electrophoresis and visualized using the Gel Doc XR+ (BioRad®). Table 1:PCR condition with two concurrent gradient setting...
plus Sybr Green I kit (Roche). qRT-PCR primers spanned at least one exon boundary where possible. The quality of qRT-PCR products was assessed using melting curve analysis and gel electrophoresis. gpd-3 RNA levels were used to normalize.©...
2DgelsGelsgelGel 系统标签: gelsimagingdecodon凝胶gel成像 Imaging 2D gels Imaging 2D gels ESSENTIAL TIPS AND TRICKS EXPLORING LIFE Imaging gels Imaging gels ESSENTIAL TIPS AND TRICKS 2D gel electrophoresis also demanding proteomic analysis and needs hard sample preparation protocol, choosing technique, an...
Then, we implemented and evaluated an image analysis protocol with 2D ImageMaster software. Using ImageMaster, different spots were identified. On two-dimensional electrophoresis gels, 40 protein spots from anionic fractions and 15 spots from the cationic fractions of urine were i...
Student’sttest.hDigitonin-solubilized isolated mitochondria from FASNlox/lox-PyMT and FASN∆/∆-PyMT MEFs in 2D and 3D culture conditions were subjected to blue native gel electrophoresis (BNGE) followed by in-gel complex I activity assay. Complex I occurs both free and bound with complex ...
General ROS generation was measured by CM-H2DCFDA following the protocol described in the literature40,41. The temperature of the medium after different plasma treatments was 25–30 °C. The ROS level in the medium without cells was elevated immediately after the treatment with different ...
Reactive oxygen species (ROS) are generated and consumed in living organism for normal metabolism. Paradoxically, the overproduction and/or mismanagement of ROS have been involved in pathogenesis and progression of various human diseases. Here, we reported a two-dimensional (2D) vanadium carbide (V2...