“disc gel electrophoresis”“圆盘凝胶电泳” 但要写: “disk drive” “磁盘驱动器” Due to the fact that 这过于冗长了。保持简洁,用because代替. Either...or 如果单独使用either作主语,必须用单数动词。例如,你可以写成: “Either buffer wor...
compared with peripheral blood mononuclear cells (PBMCs), and erythromyelogenous leukemic (K562 and K562/adr) cells.Methods: Whole cell proteomes of PBMCs, PBSCs and erythromyelogenous leukemic cells were separated by 2D-gel electrophoresis and analyzed by multivariate and principle component analysis...
摘要蛋白质组技术是植物基因功能鉴定和分析的强有力工具。经典的双向电泳技术是蛋白质组研究的核心技术，但差异蛋白质组分析使其具有更大的挑战性，因此在蛋白质水平的基因表达的定量分析研究需要更高灵敏度和更宽动力线性范围的技术。双向差异凝胶电泳（ｔｗｏｄｉｍｅｎｓｉｏｎｄｉｆｆｅｒｅｎｃｅｇｅｌｅｌｅｃｔｒｏ...
Fig. 4. Two-dimensional difference gel electrophoresis analysis of blood plasma proteins after hormonal stimulation using Ovopel and control males (PBS-treated). A) Single-channel image of blood proteins from Ovopel-treated males. B) Single-channel image of blood proteins from control (PBS-treated...
The present invention relates to the segmentation of two-dimensional gel electrophoresis images (2D images). The method according to the invention associates an initial protein seed candidate with an
蛋白质组分析的首要要求 将来自于全细胞、组织或生物体中所包含的多达几千种混合蛋白质进行分离、检测和分析。第三页，共44页。生物学问题的提出 实验模型的设计 实验组和对照组样品的制备 蛋白样品的IEF和PAGE电泳分离 图像扫描和初步分析 感兴趣蛋白点的切取 胰酶对脱色后蛋白质的消化 质谱的多肽指纹图、微...
Cycling conditions are listed in Table 1. The PCR products were detected using GelRedTM (Biotium) following agarose gel electrophoresis and visualized using the Gel Doc XR+ (BioRad®). Table 1:PCR condition with two concurrent gradient setting at denaturation and annealing steps. ...
6、el electrophoresis. Nature, 1969, 221: 1056-1057), 这即是双向凝胶电泳这即是双向凝胶电泳 (two-dimensional polyacrylamide gel electrophoresis, 2D PAGE)。 同年,同年,2D PAGE在原理上又有了新的发展,以第一向为蛋白质等电聚焦在原理上又有了新的发展,以第一向为蛋白质等电聚焦 的双向凝胶电泳技术建立...
2.3. Suspension Culture The principle of this method is to put a cell suspension inside a container and keep the cells in suspension either by agitation or by increasing the media viscosity (adding carboxymethyl cellulose) [8,25]. In the agitation-based approach, the container can either be ...
However, they were still convinced that the approach could be successful in principle but concluded that there was a need for better culture systems. In the PLATOX project presented here, the aim was to establish an unpretentious yet robust and well reproducible in vitro cell model able to ...