产品编号: 3282843 规格: ≥98% CAS NO: 2140-46-7 包装规格: 10 MG,25 MG,100 MG 产品类别: 进口试剂 品牌: Sigma-Aldrich 优惠价: 立即咨询产品价格查看更多规格... 产品编号 包装单位 单价(元) 国内现货 国外库存 询价单 3282843 10 MG 1500 3282843 25 MG 3030 3282843 100 MG 8060...
25-Hydroxycholesterol试剂CAS号:2140-46-7保存条件:-20°C产品介绍:Biologically active cholesterol metabolite上海惠诚生物为广大科研用户提供科学仪器,试剂耗材等专业科研产品服务。 代理产品品牌有:Nu-chek、LKT、AccuStandard、APSC、MPBio、Sigma-Aldrich、NIST,Reagecon、Megazyme,LGC、Ultra,日本和光(WAKO)、Shodex,...
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The slides were deparaffinized in a 60 °C oven, rehydrated, and stained with hematoxylin (Sigma). To remove over-staining, the slides were quick dipped three times in 0.3% acid alcohol, and counterstained with eosin (Sigma). They are then washed in ethanol series and xylene, and then ...
We immunoprecipitated an equal amount of chromatin with 2 μg of NFYA antibody (Santa Cruz, USA), PLAG1 antibody (Sigma-Aldrich, USA), RNA pol II antibody (Cell Signaling Technology, USA) and normal mouse IgG (provided in the ChIP Assay Kit) overnight at 4 °C. Then we collected...
ARTICLE Received 22 Dec 2015 | Accepted 6 Sep 2016 | Published 25 Oct 2016 DOI: 10.1038/ncomms13129 OPEN 25-hydroxycholesterol contributes to cerebral inflammation of X-linked adrenoleukodystrophy through activation of the NLRP3 inflammasome Jiho Jang1,*, Sangjun Park2,*, Hye Jin Hur1, Hyun-...
THP-1 cells were differentiated by treatment with 100 nM phorbol 12-myristate 13-acetate (PMA) (Sigma Aldrich). WT (catalog no. C631) and α5 integrin null (catalog no. HZGHC001085c001) human haploid (HAP1) cells, created using CRISPR-Cas9 technology were purchased from Horizon ...
系统标签: methylglutarylproteasomalmacrophagesreductasehydroxycoa Rapid proteasomal elimination of 3-hydroxy-3-methylglutaryl-CoA reductase by interferon-c in primary macrophages requires endogenous 25-hydroxycholesterol synthesis Hongjin Lu a , Simon Talbot a , Kevin A. Robertson a,b , Steven Watterson c ...
The other chemical reagents were purchased from Sigma. Measurement of anchorage-independent growth (MTT assay) Human colon cancer cell lines (DLD-1, HT-29 and KM-12) were grown in RPMI1640 medium supplemented with 10% fetal bovine serum at 37 °C in a 5% CO2 incubator. Exponentially ...
25-HC and 27-HC (Sigma–Aldrich, Dorset, UK) were all reconstituted in ethanol. 25-HC (1 µM) or 27-HC (1 µM) were added to CEF cell cultures infected with RB1B (100 pfu) at the time of infection or 24 hpi, and the cells were fixed for plaque size or harvested with ...