In 2-Deoxyglucose Assay Kit (Colorimetric), 2-DG6P is oxidized to generate NADPH, which is determined by an enzymatic recycling amplification reaction. This easy to use non-radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/sample.Catalog Number KA...
检测原理: 细胞摄入2DG后,经过细胞内生化酶的催化,通过检测产生的NADPH进行研究。 所有反应步骤都只需在同一个孔内添加预混试剂,即可实现自动化操作。 ●为减少葡萄糖代谢的影响,只需少量2DG加入活细胞中。通过细胞代谢将2DG转化转化为2DG6P。 ●2DG6P在被氧化的过程中会产生NADPH,通过检测NADPH的量来测定2DG6P...
询价记录 相关实验 货号:CSR-OKP-PMG-K01TE 供应商:上海金畔生物科技有限公司 营养摄入 温馨提示:不可用于临床治疗。 风险提示:仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。
Males exposed to females (regardless of whether they courted or not) showed widespread, non-specific increases in 2-DG uptake relative to males not exposed to females. The results indicate the utility of the 2-DG technique for studying complex, species-typical behaviors in vertebrates....
A method for measuring local rates of glucose uptake in mammalian brain tissue 1,2 has been used to study monkey cortex receiving visual stimulation through one eye 3 . The resulting pattern of glucose uptake resembled that of the ocular dominance columns, identified by several independent methods...
Activation of protein kinase C by phorbol esters caused a time- and dose-dependent stimulation (270% of control) of glucose uptake in cultured glia, but not in neurons from rat brain. The phorbol ester stimulation of Vmax of glial glucose uptake occurred only in glia despite nearly 2.5-fold...
The effects of early onset, unilateral conductive hearing loss on tone-induced 2-deoxyglucose (2-DG) uptake in the auditory cortex of juvenile Mongolian gerbils (Meriones unguiculatus) were studied. Atresia of the left ear canal was induced at postnatal day 9 (P9) to achieve reversible auditory...
2-deoxyglucose, trapping the product 2-deoxyglucose-6-phosphate intracellularly (with exception of liver and kidney)[citation needed]; thus, labeled forms of 2-deoxyglucose serve as a good marker for tissue glucose uptake and hexokinase activity. Many cancers have elevated glucose uptake and ...
The uptake of [14C]2-DG during silence was compared with that observed during exposure to wide band noise (WBN) or pure tones at an intensity level of 85 dB SPL. In silence, the highest levels of 2-DG uptake were observed in the spiral ligament, spiral prominence and stria vascularis, ...
Nonradioisotope assay of glucose uptake activity in rat skeletal muscle using enzymatic measurement of 2-deoxyglucose 6-phosphate in vitro and in vivo. Biol Signals Recept. 2000 Sep-Oct;9(5):267-74.Miwa H, et al. Leukemia cells demonstrate a different metabolic perturbation provoked by 2-...