1) cell stage 2-细胞期 2) 2 cell stage embryos 2-细胞期胚卵 3) cell cycle Bcl-2 细胞周期Bcl-2 4) cell division cycle 2 细胞分裂周期2 5) mouse two-cell embryos 小鼠2-细胞期胚胎 1. Aim: To explore the effect of Cdc25B overexpression on the development ofmouse two-cell embryos. ...
The developmental ability of blastomeres of late 2-cell stage embryos fused to chemically enucleated oocytes of different ages or cytoplasts from different sources was also examined in vitro. Metaphase I oocytes cultured in different concentrations of ETO-CHXM (10-50 micrograms/ml/each) extruded ...
When the constructed embryos developed to the 2-cell stage in KSOM-AA medium (Sigma, St Louis, MO, USA), they were transferred to the oviduct of pseudopregnant ICR mice at 0.5 d.p.c. Comparative genomic hybridization analysis The genomic DNA of wild-type and H19Δ AG-haESCs was ...
Mouse embryos at the 2-cell stage were cultured in the presence of cytochalasin B (CB), cytochalasin D (CD), colchicine (COL) or colcemid (COM) for up to 72 h. Cleavage was arrested in the 2-cell and 8-cell embryos cultured in CB or CD but the blastomeres continued to differentiate...
2022年3月,由中国科学院和深圳华大生命科学研究院牵头的体细胞诱导培养人类全能干细胞研究在Nature上发文“Rolling back human pluripotent stem cells to an eight-cell embryo-like stage”。在这项研究中,科研团队开发了一种非转基因、快速且可控的方法,从人类多能干细胞中诱导产生8C样细胞(8C-like cells,8CLC...
适配显微镜 倒置显微镜,如Confocal、TIRF、相差、高内涵、 超高分辨PALM、多光子显微镜等,需配置96孔板stage holder 成像窗口材料 170μm玻璃片 微流控培养板尺寸 参照96孔标准板型 细胞培养室数量 4个 细胞培养时间 常规持续培养1-3天,超长可达两周时间 样本体积 5-10μl细胞悬浮液(动物细胞) 50μl细胞悬浮液...
(1C) stage and late 2-cell (2C) stage in mice, respectively.3By the 8-cell (8C) stage, blastomeres could still contribute to all lineages in chimeric mice4,5,6and were considered as totipotent states in a broad definition.7At the late 8C stage, the mouse embryo starts to compact ...
Introduction GEfetch2Ris designed to accelerate users' downloading and preparation of single-cell/bulk RNA-seq datasets from public resources. It can be used to: Forbulk RNA-seq: Downloadraw data (sra/fastq/bam)fromSRA/ENAwithGEO accessions: ...
In addition to showing similar transcriptome features, 2C-like cells exhibit chromatin architecture resembling that of the 2C stage (Eckersley-Maslin et al., 2016; Ishiuchi et al., 2015). In addition to their unique molecular signature, 2C-like cells show an expanded ability to contribute to...
Traumatic brain injury (TBI) is the leading cause of mortality and disability worldwide1,2. TBI develops as a two-stage process. The first stage is a mechanical injury of brain tissue that directly leads to neuronal death. At present, surgery is the first choice for treatment during this st...