6.4.2 电泳 [1] 在待测样本中加入等量的 2×SDS 蛋白上样缓冲液,100℃加热 5min; [2] 每孔上样 20μl; [3] 浓缩胶上所加电压为 80V,约 15～20min; [4] 当染料前沿进入分离胶后,把电压提高到 120V; [5] 继续电泳直至溴酚蓝到达分离胶底部,然后关闭电源; [6] 下胶。转膜 6.5.1 转膜缓冲液...
COURSE:History 101"Introduction to American History"INSTRUCTOR:Dr,Jane KlammerOFFICE:305Marshall HallOFFICE HOURS:11:15～12:30M W F(Monday Wednesday Friday)CLASS:363Marshall Hall 3:35～5:00T Th (Tuesday Thursday)10:10～11:00M W F Other times by appointmentTELEPHONE:...