Treatment of cultured fibroblasts, designated HA I cells, with 4-hydroxy-2-nonenal (4HNE) in doses up to 50 nmol/106 cells for 3 h results in dose-dependent cytotoxicity measured by clonogenic cell survival with 50% cytotoxicity achieved at 32 nmol 4HNE/106 cells. 4HNE treatment also ...
26. Mechanisms of 4-hydroxy-2-nonenal induced pro- and anti-apoptotic signaling. [PMID: 20565132] 27. Metabolic Preconditioning of Mammalian Cells: Approaches for Increasing Biostability after Desiccation. 28. Metabolic Flux Distribution during Defatting of Steatotic Human Hepatoma (HepG2) Cells. [...
Indeed, preincubation of cells with HNE and their subsequent treatment with H2O2 or MMS increased the number of nuclear poly(ADP-ribose) foci, known to appear in cells in response to SSBs. However, when purified BER enzymes were exposed to HNE, only ANPG and TDG glycosylases excising ɛA...
and endogenous 4-hydroxy-2-nonenal (4-HNE) and malondialdehyde generated by polyunsaturated fatty acid peroxidation1,2. TheALDH2rs671 single-nucleotide polymorphism in exon 12 has received much research attention. This variant, G to A in the gene ...
ALDH2 is a tetrameric enzyme responsible for the oxidization of aldehydic substrates, including those generated during oxidative stress such as 4-hydroxy-2-nonenal.7 We have previously found that the inability of ALDH2 to remove endogenous cytotoxic aldehydes accumulated during oxidative stress is in...
1a). We then immunohistochemically detected protein adducts with 4-Hydroxy-2-nonenal (HNE), a major component of lipid peroxidation, which were greater in the liver of both cholestasis and steatohepatitis models; of note, the HNE-protein adducts were mainly detected in the portal tracts (where ...
反-2-壬烯醛(CAS:18829-56-6;英文名:trans-2-Nonenal;)实验注意事项: 1.使用18829-56-6实验前需戴好防护眼镜,穿戴防护服和口罩,佩戴手套,避免与皮肤接触。 2.实验过程中如遇到有毒或者刺激性物质及有害物质产生,必要时实验操作需要手套箱内完成以免对实验人员造成伤害。
nonenal (4-HNE), αM, phospho-ERK1/2, ERK1/2, phospho-Src, Src (Cell Signaling Technology, Danvers, MA, USA), p47phox(1:1,000; EMD Millipore, Temecula, CA, USA), gp91phox(1:1,000; BD Transduction Laboratories, San Jose, CA, USA), and GAPDH (1:1000, Abcam, Cambridge, MA,...
Neuronal cells can internalize HNE-modified alpha-synuclein oligomers, and HNE treatment increased the cell-to-cell transfer of alpha-synuclein proteins. Innovation and Conclusion: These results indicate that HNE induces the oligomerization of alpha-synuclein through covalent modification and promotes the ...
The transforming growth factor beta-induced gene (TGFBI; BIGH3; βigh3) was first discovered in a human lung adenocarcinoma cell line, where it was upregulated after treatment with the pleiotropic cytokine TGF-β1 (Skonier et al. 1992). TGFBI mRNA encode