D-MEM/Ham's F-12 + 20% SSR + 2mmol/l L-Glutamine +1×MEM Non-essential Amino Acids + 0.1mmol/l StemSure® 2-Mercaptoethanol + 1×Penicillin-Streptomycin + 5ng/ml bFGF (2) 未分化标记的发现 使用StemSure®Serum Replacement(SSR)培养的人iPS细胞201B7株,确认各种未分化标记(Sox2, Oct...
Material Safety Data Sheet(MSDS) Back Directory [msds information]2-Hydroxy-1-ethanethiol(60-24-2).msds Spectrum Detail Back Directory [Spectrum Detail]2-Mercaptoethanol(60-24-2)MS2-Mercaptoethanol(60-24-2)1HNMR2-Mercaptoethanol(60-24-2)13CNMR2-Mercaptoethanol(60-24-2)IR12-Mercaptoethanol(60...
The cells were collected by centrifugation at 1600 rpm and lysed in a lysis buffer containing 1% 2-mercaptoethanol. Then, 70% ethanol was added to the lysate in a 1:1 ratio; the lysate was transferred to the spin cartridge with a collection tube and centrifuged for 15s at 12,000 g. ...
Usually derived from Scatchard plot of binding data. Analogous to Vmax in enzyme kinetics. BME (1) Beta-mercaptoethanol (βME). (2) Engineered Arabidopsis lines that exhibit beta-glucuronidase expression in the micropylar end of the seed, named Blue Micropylar End, BME lines. Bmf A pro-...
The splenocytes were washed once with cold PBS and resuspended in culture medium (RPMI containing 10 % fetal bovine serum, 1 % Penicillin and Streptomycin, 1 mM sodium pyruvate, 10 mM HEPES and 50 µM 2-Mercaptoethanol). To quantify cytokine production in cell culture supernatants of re-...
GBP2 (GFP-mGBP2) or GFP alone11 were cultured in Dulbecco's modified Eagle's medium (DMEM, Invitrogen/Gibco) supplemented with 10% (v/v) heat-inactivated low endotoxin fetal bovine serum (FBS, Cambrex), 2 mM L-glutamine (Biochrom) and 0.05 mM β-mercaptoethanol (Invitrogen/ Gibco...
Peptide was placed under vacuum and precipitated by 2-Mercaptoethanol in a 55° C. water bath under stirring. After filtering through dampened Celite, the filtrate was rotary evaporated, acidified with 0.1% TFA in water and lyophilized. Biological Procedures Immunizations and Animals. Female New ...
Then, 15 μl of SDS sample buffer with 2-mercaptoethanol for reduction was added to an equal amount of samples of cultured medium or lysates. These samples were boiled at 95 °C for 5 min, subjected to SDS-polyacrylamide gel electrophoresis with 10% acrylamide separating gel (e-PAGEL; ATTO...
mercaptoethanol, and 20% v/v glycerol. For PLpro purification, an additional step was performed after elution with buffer B, where the protein was cleaved by the SUMO protease (ULP1) to remove the N-terminal SUMO tag before loading onto the size-exclusion chromatography column....
Cell lysates were collected at 24- and 48-hpi in the presence of Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific) using 1x lysis buffer (2% SDS (w/v), 10% glycerol (v/v), and 1% β-mercaptoethanol (v/v), 160 mM Tris-HCl, pH 6.8) and boiled for 10 min...