by the role of phosphofructokinase as the rate-limiting step of glycolysis33. As 2DG inhibits the two prior reactions catalyzed by hexokinase and phosphoglucose isomerase44,45, lower levels of fructose-6-phosphate would lead to even slower production of fructose-1,6-bisphosphate compared to later ...
Following euthanasia, the pancreas was promptly excised and immersed in HEPES buffer solution (pH 7.25–7.35; 140 mM NaCl, 4.7 mM KCl, 10 mM HEPES, 10 mM glucose, 1 mM CaCl2, and 1.13 mM MgCl2). Subsequently, injecting 250 U of collagenase (C9697, Sigma-Aldrich) into ...
n = 6. Different letters indicate significant difference between groups. P-value ≤ 0.05 is considered as significant. F-1,6-BP, fructose-1,6-bisphosphate; DHAP, dihydroxyacetone phosphate; G-6-P, glucose 6-phosphate; 6-PG, 6-phosphogluconate; G-3-P, glyceraldehyde 3-phosphate; ...
Following euthanasia, the pancreas was promptly excised and immersed in HEPES buffer solution (pH 7.25–7.35; 140 mM NaCl, 4.7 mM KCl, 10 mM HEPES, 10 mM glucose, 1 mM CaCl2, and 1.13 mM MgCl2). Subsequently, injecting 250 U of collagenase (C9697, Sigma-Aldrich) into ...