CAGGAAGTAAGATGCCTGGAAC 下游:TGCAGCAGTACCAAGTTTAGTG 上游:AAGAGCAGCATGACCTCTCAC 下游:CTGCCTCAAGTCAGTGCCT 上游:AGAGAGCGATGCAGGAAACTT 下游:AAGGATGCCCACATCTTTTGG 上游:AAGAGGGATGCTGCCCTTAC 下游:TACGGCCAAATCCGTTCACA 筛选标准.使用 Cytoscape 软件构建"基因-中医症 状/现代医学症状"和"基因-中药"关联...
FDX1 上游:CCTCTTTGGAGTCTCTCGCGG 下游:CAGCCCAACCGTGATCTGT DLD DLAT GAPDH 上游:CTCTTGCTTTGGAATATGGAGC 下游:AAAAGCTTCTGATAAGGTCGGA 上游:ACTCCCCAGCCTTTAGCTC 下游:CAATCCCTTTCTCTACTGCCAAC 上游:GGAGCGAGATCCCTCCAAAAT 下游:GGCTGTTGTCATACTTCTCATGG dehydrogenase,GAPDH)为内参按 2−∆∆Ct 法分析....
下调HMGB2表达对肝癌LM3细胞上皮-间质转化的抑制作用及 其AKT/mTOR信号通路机制 魏雁虹1, 杨晨雪1, 杨广民1, 宋帅1, 李明1, 杨海娇1, 魏海峰2 （1. 吉林省人民医院医学检验中心，吉林长春130021；2. 吉林省人民医院生物治疗与基因诊断 重点实验室，吉林长春130021）［摘要］目的 目的：探讨下调肝癌细胞中高...
and cDNA was synthesised with a Rever Tra Ace qPCR kit (Catalogue Number FSQ-201, TOYOBO, Japan). qRT-PCR was conducted using SYBR Green Real-Time PCR Master Mix (Catalogue Number QPK-201, TOYOBO, Japan) on a CFX96 Touch Detection System (Bio-Rad, USA). The 2−∆∆Ctmethod...
The human ACE2 transcript variant 2 was amplified using the OriGene primer set Forward: 5’-TCC ATT GGT CTT CTG TCA CCC G-3’ and Reverse: 5’-AGA CCA TCC ACC TCC ACT TCT C-3’. HPRT was used as the control housekeeping gene using primers, Forward: 5’-TCA GGC AGT ATA ATC CAA...
The sequences of the BAX primers were: 5’-TGGCAGCTGACATGTTTTCTGAC-3’ and 5’-TCACCCAACCACCCTGGTCTT-3’ , while the sequences of the GAPDH primers were: 5-ATGGGGAAGGTGAAGGTCG-3’ and 5’-GGGTCATTGATGGCAACAATATC-3’ , resulting in a 107-bp PCR amplicon. qRT-PCR was ...
(F: 5-’ TCAGGCGTCTGTAGAGGCTT-3’R: 5-’ ATGCACATCCTTCGATAAGACTG-3′), ZEB1 (F: 5-’ GATGATGAATGCGAGTCAGATGC-3’R: 5-’ ACAGCAGTGTCTTGTTGTTGT-3′), ZEB2 (F: 5-’ CAAGAGGCGCAAACAAGCC-3’R: 5-'GGTTGGCAATACCGTCATCC-3′), Vimentin (F: 5-’ GACGCCATCAACACCGAGTT-3’R...
4304970 β2M-246F TGACTTTGTCACAGCCCAAGATA 58.4 43 4304970 β2M-330R AATCCAAATGCGGCATATTC 57.2 40 450025 β2M-275P VIC-TGATGCTGCTTACATGTCTC GATCCCA-TAMRA 68.2 48To correlate c-erbB2 protein expression with c-erbB2 gene amplification, tissue sections from 10 cases (6 DCIS and 4 IDC) wi...
ifseraetntrciebueftfeedcttsowthheicfhacctatnhaetxtphleailnowthliesvdeelcorfepas-epoinlaχrixzIxTezAd, see Supplementary Fig. S5(a). The large variation generated SH power for s-polarized fundamental bofeaχmzIxTxiAs close to the detection limit. Note that the visibility of the fringes ...
Second, weak localization is also dethuwhxpeiigectrthhliusuvrcFdednhdSiefesdfaamei,ngrnbeneiesensagocttsatauhsrtuirohovespaweepcyLρn.aeTMnsxix.onb(BRTtetee,h)scbidetacuuteoithnnsnteethsfioeretftiohreMsmledetRds.ooTitforFoheFfiicgFSRrt.edi a1r3o4,nm,en3ni2.seioioI-gsnfltaiprcqtotouiu...