(16S library preparation and sequencing. 16S rRNA gene libraries targeting the V4 region of the 16S rRNA gene were prepared by first normalizing template concentrations and determining optimal cycle number by way of qPCR. Two 25 µL reactions for each sample were amplified with 0.5 units of Phu...
宏基因组测序(Metagenomics Sequencing)是对环境样品中全部微生物的总DNA进行高通量测序,主要研究微生物种群结构、基因功能、微生物之间的相互协作关系以及微生物与环境之间的关系。宏基因组测序研究摆脱了微生物分离纯培养的限制,扩展了微生物资源的利用空间,为环境微生物群落的研究提供了有效工具。 高通量测序平台 16S扩...
[2] Franzén, Oscar, et al. 'Improved OTU-picking using long-read 16S rRNA gene amplicon sequencing and generic hierarchical clustering.' Microbiome 3.1 (2015): 43. [3]Bowman, Brett, et al. 'Analysis of full-length metagenomic 16S genes by SMRT sequencing.' Chemistry 4 (2013): C2. MAGI...
结论 :16S rRNA基因序列分析是一种有效的病原细菌鉴定方法。 【关键词]16S rRNA;病原细菌 ;基因序列分析 Identification of pathogenic m icroorganism by sequencing I 6S rRNA gene ZHU Feizhou ,CHEN Liy1J2J CHEN Hanchun Department ofBiochemi stry,School ofLi fe Sciences;2.Department ofMicroorganism,...
The development of 16S rRNA gene sequencing technology was briefly introduced. Based on the research results of 16S rRNA gene sequencing technology in drinking water, the relationship between water environmental conditions and microbial diversity in the whole process was discussed. The results showed ...
16S rDNA是细菌染色体上编码 rRNA相对应的DNA序列，存在于所有细菌染色体基因中。该序列包含9个高变区和...
鉴定rrna杆菌ochrobactrum致病pathogenic ·基础实验研究论著·16SrRNA测序鉴定1株条件致病性人苍白杆菌*洪文艳,李曦,刘建伟,于德宪,谢晓波,刘金华,唐博恒△(广州军区疾病预防控制中心疾病监控科,广东广州510507)摘要:目的探索一种基于16SrRNA基因的细菌快速鉴定方法,为临床未知病原菌的诊断及治疗提供科学依据。方法对临床患者...
文章探究了一类新型产甲烷古菌(Ca. Methanoliparum)的代谢途径并证实了其独立降解长链烷基烃产甲烷的功能,在古菌厌氧降解长链烷烃产甲烷研究方面取得突破性进展。其中,16S rRNA微生物多样性、宏基因组和宏转录组测序工作均在诺禾致源完成。 下面,小编带大家一起学习下这篇高分文章:...
Sequencing Datasets (16S rRNA gene) How data were acquired 16S rRNA next-generation sequencing of extracted DNA from human skin microbiome swab samples.Instrument: Ion Torrent PGM at MRDNA/Molecular Research LP [1]Software: QIIME [2], PICRUSt [3] Data format RawAnalyzed Parameters for data ...