(We surveyed bacterial and archaeal diversity using amplicon sequencing of the 16S rRNA gene, a common taxonomic marker for bacteria and archaea12 that remains a valuable tool for microbial ecology despite the introduction of whole-genome methods (e.g., metagenomics) that capture gene-level function...
16S rRNA 是一种参与制造原核核糖体小亚基的 rRNA;它们是 30S 亚基(原核核糖体)的组成部分。 一般来说,它具有与较大亚基中的 rRNA 类似的结构功能,将核糖体的蛋白质保持在固定位置。它们还参与通过与较大亚基中的 23s rRNA 相互作用来促进小亚基与较大亚基的融合。古细菌、细菌、叶绿体和细菌中的小核糖体单元含...
在16S rRNA基因测序中，扩增子（amplicon）的大小是一个重要的参数，因为它影响测序的质量和后续的数据分...
(B) The 2D and 3D structure of the 16S rRNA gene. Individual regions are shown in the same color (Yang et al. 2016). (C) The regions and primers of 16S rRNA. Service you may intersted in 16S/18S/ITS Amplicon Sequencing Absolute Quantitative 16s/18s/ITS Amplicon Sequencing Full-Length...
16S rRNA gene sequencingBioactive compoundsPiperBioprospectingPiper longum (also known as Indian long pepper) is widely used in Ayurvedic, Siddha and Unani medicine systems. The principle bioactive compound of this plant is piperine, which mainly accumulates in the fruits called spikes. The report of...
Gut microbiota affects health, metabolism and immunity of the host, and in the case of livestock, also food-safety. Here, 16S rRNA gene high-throughput Illumina sequencing was used to describe the microbiome of chicken caeca in two different breeds and m
We performed amplicon-based 16S rRNA gene sequencing of DNA extracted from swab samples collected from dairy cows affected with IH, infected IH, IP, and complicated SU, TN, and WLD lesions. DNA was also collected from healthy skin control swabs and was also analysed for comparative purposes. ...
原文检索 Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary 原文链接:https://mp.weixin.qq.com/s/4n6UzXAwE7kl3wdvCZpwDw 详情点击:https://mp.weixin.qq.com/s/R2w9O8R_Aeudv1pX6p80jg
[8] TamakiH, Wright CL, Li X, Lin Q, Hwang C, Wang S, Thimmapuram J, Kamagata Y, Liu W T., (2011) Analysis of 16S rRNA amplicon sequencing options on the roche/454 next-generationtitanium sequencing platformPloS One, 6(9) : e25263. ...