1.10x Barcoded Gel Beads：a. 这些是带有独特条形码的凝胶珠，每个Gel Beads上都有一个特定的条形码...
即上图的“10X Barcoded Gel Beads”。每一个凝胶珠有足够多的人工设计序列附着。其序列组成如下图所...
10x Genomics具有通量高、灵活性强、流程简单、技术性能高、单个细胞制备成本低等优势,其技术核心是油滴包裹的凝胶珠(GEM),该系统有75万种barcoded beads,每个bead上有40-80万探针。 技术要点 > 8通道的微流体“双十字”交叉系统 >含Barcoded RT Primers的Gel Beads、细胞和酶的混合物、油 > 三者结合形成GEMs...
10x Genomics具有通量高、灵活性强、流程简单、技术性能高、单个细胞制备成本低等优势,其技术核心是油滴包裹的凝胶珠(GEM),该系统有75万种barcoded beads,每个bead上有40-80万探针。 技术要点 > 8通道的微流体“双十字”交叉系统 >含Barcoded RT Primers的Gel Beads、细胞和酶的混合物、油 > 三者结合形成GEMs...
Each GEM is the site of a micro-reaction, in which Gel Beads are dissolved and target molecules from each cell are captured and barcoded, marking each target molecule's cell of origin. The Chromium instrument advantage Did you know? Chromium X series instruments automate the most critical step...
技术原理与流程 GEMs制备(Gel Beads-in-emulsion) 制备好的细胞悬液,10x barcode Gel beads (试剂根据研究目的不同而使用不同试剂盒)和油滴分别加入到芯片中经由微流体 "双十字"交叉系统形成GEMs. 单细胞转录组测序 ① 3'端带有polyA的RNA被逆转录为带有10x Barcode和UMI信息的cDNA一链 ② 磁珠纯化一链cDNA,...
10x Genomics Chromium Workflow说明书 10x genominics單細胞定序平台介紹-從原理應⽤到分析 莊景凱Ph.D 微⽣物體研究中⼼（BMRC)圖爾思⽣物科技股份有限公司
barcode from a pool of 750,000. After HiSeq sequencing the barcoded short-reads can be assembled into contiguous sequences averaging 50kb in length. Just 1ng of input DNA is split across 100,000 gel-coated beads (GEMs), with processing completed in 5 minutes. Samples are pooled for ...
Each Gel Bead, infused with millions of barcoded oligonucleotides, is mixed with a sample, which can be high molecular weight (HMW) DNA, individual cells, nuclei, or Cell Beads. Gel Beads and samples are then added to an oil-surfactant solution to create Gel Beads in EMulsion (GEMs), ...
10x Genomics仪器小巧,但功能强大,具有通量高、灵活性强、流程简单、技术性能高、单个细胞制备成本低等优势,其技术核心是油滴包裹的凝胶珠(GEM),该系统有75万种barcoded beads,每个bead上有40-80万探针。 利用8通道的微流体“双十字”交叉系统,将含barcode的凝胶珠(Gel Beads)、细胞和酶的混合物、油三者混合,形...