pylori SS1 (1 × 107 CFU/ml) for 14 days during each cycle. Three cycles of treatment were carried out to establish GC mice model. After 180 days of treatment, mice were scarified for subsequent analysis. The tumor size per stomach were counted (n = 10 per group). Murine ...
reuteri 1 × 108 CFU/ml for 2 h (37 °C, 5% CO2). In the SE PT4 infected group, explants were treated with culture medium containing Salmonella Enteritidis PT4 at a concentration of 1 × 107 CFU/ml, incubated for 2 h (37 °C, 5% CO2). In the pre-treatment experimental...
The capability to supply exopolysaccharides was screened in chosen lactic acid micro organism (LAB) isolates. Production of natural acids in the spontaneous fermentation dropped the pH to 4.0 ± 0.3. The group of presumptiveLABreached 8.37 ± 0.01 log colony forming models (CFU)/mL by day 8 of...
We added 2.5% L.P dy-1 (4 × 108 cfu/mL) to the barley flour with seven-times deionized water to ferment at 30 ℃ for 24 h. After centrifugation (8, 000 ×g for 20 min), the supernatant of the fermented liquid was dried with vacuum freeze drier (Marin Christ, Germany) and ...
盆栽试验结果显示，添加108 cfu/mL 真菌，土壤pH 值比对照显著降低了0.35个单位；对碎米荠幼苗生长无不良影响，平均株高为7.4 cm ，平均根长为9.6 cm ，平均生物量为2.9 g ，均高于对照；土壤中有效硒的含量显著提高，活硒率最高达到2 4.8%，而有效镉的含量显著降低，抑镉率达到24.7%；碎米荠地上/...
Under optimized conditions, the peak current response of the LIP sensor showed a linear relationship with the logarithmic value of LGG concentration in the range from 101 to 109 CFU mL−1 and a detection limit of 5 CFU mL−1. The proposed LIP sensor has achieved efficient, ultrasensitive,...
Using quantitative ELISA, we determined the amount of MPER produced by the modified EcN to be 14.3 µg/108 cfu. Conclusions The CRISPR/Cas9 technology was an effective method for establishment of recombinant EcN-MPER bacteria that was stable over many passages. The developed EcN-MPER clone ...
The DrNLRP3 inflammasome occurrence is optimized at 6 hpf embryos or 72 hpf larvae with Edwardsiella tarda immersion infection (108 CFU/ml) for 1–4 h as determined by significantly increased DrCaspase-A/-B activity at those times (Fig. 9, A and B). DrNLRP3 overexpression alone in ...
All the assays were performed at least three times. To compare the expression of RBG1, WRKY45, and PR2 between KO and RBG1res-NIL after inoculation with B. glumae, both seed types were soaked in a bacterial inoculum suspension whose concentration was adjusted to 108 CFU/ml with ...
(3)GES-1细胞与Hp共同培养,取处于对数生长期的GES-1细胞0.25%胰酶消化后进行细胞计数,将1×106个GES-1细胞铺于底面积为25 cm2细胞的培养瓶中,用无抗生素含10%FBS的RPMI-1640培养基常规培养过夜,次日晨用无抗生素含10%FBS的RPMI-1640培养液调节Hp浓度为1×108CFU/mL,按细胞与细菌比例为1:1分别加入Hp,置于...