After exposure, the membrane was stripped in boiling stripping buffer (0.1% SDS and 5 mM EDTA) and re-hybridized using a GAPDH probe (5′-GTAGACCCACGACATACTCAGCACCGGCCTCACCCCATT-3′) as a loading control. RNA-
Cells were treated with 50 μM rapamycin (Cayman Chemical) for 3 h. For phosflow, Fixable Viability Dye eFluor 780 (65-0865-14, eBioscience) was used prior to fixing with Cytofix (554655, BD Biosciences). Cells were permeabilized with perm buffer III (558050, BD Biosciences) and stai...
在提及G1的垃圾收集过程时,需要理解几个G1的重要的分区内部的详细数据结构、以及核心算法。 TLAB(Thread Local Allocation Buffer)本地线程缓冲区 G1 GC会默认会启用Tlab优化。其作用就是在并发情况下,基于CAS的独享线程(mutator threads)可以优先将对象分配在一块内存区域(属于Java堆的Eden中),只是因为是Java线程独...
However, the user won't notice it if 0.1 percent of the program becomes three times faster. Here's a third approach that I used to make my code run faster, back in the days of JDK 1.3. Instead of creating an empty StringBuffer, I sized it to the number of required characters, like...
2.1.7. Usage of _bufferSize There is no cudaMalloc inside cuSolver library, the user must allocate the device workspace explicitly. The routine xyz_bufferSize is to query the size of workspace of the routine xyz, for example xyz = potrf. To make the API simple, xyz_bufferSize follow...
STM32是STMicroelectronics推出的一款基于ARM Cortex-M内核的微控制器系列,广泛应用于嵌入式系统开发。串口通信是嵌入式系统中常见的通信方式之一,可用于与外部设备、传感器、无线模块等… 对error视而不见 使用Keil下载Hex文件进STM32 前言 初学STM32时,是通过串口1把Hex文件下载进STM32的,需要一个串口模块,而且还要...
Note that the backtrack sequence is given in reverse logical order: if the current glyph is at position i in the text glyph buffer, the backtrack sequence begins at i-1 and decrements offset values moving away from i. The lookahead sequence begins after the input sequence and increases in...
The next day, the samples were washed by the wash buffer 3 times for 10 min per time. Then, the tissues were incubated with secondary antibodies and DAPI for 2 hours at room temperature. After performing three washing steps, fat body were mounted onto slides. Primary antibodies used here ...
在DMA单次传输模式下,DMA传输完指定的数据后,DMA停止传输,而ADC还在不断采集数据,但后续采集的数据没有更新到Buffer中。如果不加此行,那么串口UART4只打印一组就数据,后续打印的数据也还是前面ADC第一组转换得到的值,此值不再更新,那么必须加此行,目的是再次启动ADC请求DMA传输; 第68~80行的代码是适用于DMA...
番茄幼苗根部转移含WSP-1(15 µM)的20 mM Hepes-NaOH buffer(pH 7.5)。25℃避光孵育40min,根部用蒸馏水清洗后立即用荧光显微镜检测(Ex/Em=465/515nm)。文献来源:Li YJ et al. In site bioimaging of hydrogen sulfide uncovers its pivotal role in regulating nitric oxide-induced lateral root formation....