(BSA), 50 mM KCl and 2 mM ATP) at 37 °C for 10 min. The reaction mixtures were fractionated in 2% agarose gels in TAE buffer (40 mM Tris, 20 mM acetate pH 7.5, and 2 mM EDTA) at 4 °C. The
3. 本产品已保存在1xLoading Buffer 中,可直接用于电泳,使用方便。4. 本产品不能用于聚丙烯酰胺凝胶电泳。推荐电泳缓冲液及琼脂糖凝胶浓度:本产品推荐使用1xTAE电泳缓冲液,推荐琼脂糖浓度:1.0%~1.5%,小片段电泳,建议使用GelRed核酸染料。使用说明1. 制备含核酸染料的琼脂糖凝胶,如EB或者GelRed。2. 凝胶浓度对...
Given the different sizes of circRUNX1 and linear RUNX1 mRNA, cDNA (complementary DNA) and gDNA (genomic DNA) samples were separated using 2% agarose gel electrophoresis with TAE buffer. DNA was separated by electrophoresis at 130 V for 45 min. Super DNA Marker (CWBIO, Beijing, China)...
The constructed sensor demonstrated the superior detection efficiency towards the oxidation of AMT in phosphate buffer solution (PB, pH =7) with a broad linear range from 0.01 μM to 1 μM, a high sensitivity of 58 µA/µM cm2, and a low limit of detection of 2.39 nM. The ...
Aliquots of the PCR products obtained were also analyzed by aga- rose gel electrophoresis using 1.5% agarose gels in 1 × TAE buffer (40 mM Tris, 20 mM Acetate, 1 mM EDTA, pH 8.6), and used for cloning into the pJET1.2 plasmid as described below. RT‑qPCR. All RT-qPCR...
(DPV) method. The decrease in the guanine oxidation peak current at +0.82 V was used as an indicator for the interaction in 0.5 mol L−1acetate buffer (pH 4.8) containing 0.02 mol L−1NaCl. Under the optimal conditions, the guanine oxidation peak currents were linearly proportional to ...
Agarose gel electrophoresis Dilute 50 × TAE to 1 × , configure a 2% agarose gel, and then immediately add EB dye and mix. After cooling, 5 μl of qRT-PCR product and 1 μl of loading buffer were added and mixed, and electrophoresis was performed at 110v–120v for ...
This option may be specified multiple times. -r rmname Specifies the resource manager associated with this server. The value rmname must appear in the resource manager table located in $TUXDIR/udataobj/RM. Each line in this file is of the form: ...
Beads were washed five times in an IP buffer. Washed beads were directly resuspended in Sample Buffer Laemmli 2x (Sigma S3401), and boiled at 95 °C for 10 min. DNA–FISH on metaphase spreads DNA–FISH was performed as previously described32,38. Slides were treated with RNase A for...
For selected experiments, PCR products were run in TAE buffer on 2% agarose gels and visualized with SYBR Safe DNA (Invitrogen). Intestinal organoid culture Small intestinal organoids were generated following published protocols22, and as previously described64. Briefly, intestinal organoids were grown...