Subsequently, 10 µL of 50% poly(U)-agarose (Sigma Aldrich) in binding buffer was added to each reaction mixture and incubated for 30 min at 4 °C. The beads were washed six times in binding buffer following centrifugation for 1 min at 4 °C. Finally, proteins bound to the poly(U...
Then, 100 μL of 1x binding buffer was used to suspend the cells. Cells were stained with 5 μL of Annexin V-FITC (Catalog: A211; Vazyme, Nanjing, China) and 5 μL of PI staining solution at room temperature for 10 min. Apoptosis was analyzed by flow cytometry and ...
is available to represent the same memory that the specified IBuffer interface represents. You can then read the bytes from this stream. Keep in mind, however, that you’re now working on the bytes directly—that is, the byte array is four times larger than the integer array returned from...
--ignore-times 这意味着rsync将检查每个文件的和,即使时间戳和文件大小匹配。这意味着它将同步比默认行为更多的文件。kvm vncvnc viewer连接kvm虚拟机连接不上的解决方法 https://blog.csdn.net/kepa520/article/details/50329505video vga里变成了qxl,调回vgarsync “Operation not permitted” https:/...
40 NativeMemoryArray Utilized native-memory backed array for .NET and Unity - over the 2GB limitation and support the modern API(IBufferWriter, ReadOnlySequence, scatter/gather I/O, etc...). Cysharp 156 41 github-actions-for-desktop-apps This repo contains a sample WPF application to demonst...
The binding of Rat2 cells nuclear proteins to the P1 minimal promoter region was confirmed by EMSA analysis. We also showed the existence of a minimal promoter region within the sequence -720/-537 upstream the ATG for transcript T2 and a strong positive regulation region in the exon E-3. ...
Cells were fixed with 4% formalin in phosphate buffer for 30 min at room temperature and then stained with 0.6% (w/v) filtered oil red O solution (60% isopropanol, 40% water) for 30 min. Then, the cells were washed with dH2O for three times and photographed under a Leica DMR XA flu...
The matrix beads were washed three times with immunoprecipitation buffer and twice with 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, and 5% glycerol. All of the wash steps were performed at 4°C for 10 min on a rotary shaker followed by centrifugation at 1500 rpm. The immunoprecipitated proteins...
(NAC) transcription factors; anddehydration responsive element binding protein(DREB) andlate embryogenesis abundant(LEA) proteins) was found to change in the transgenic plants when compared with that in the wild-type plants (Table2). Therefore, the enhanced drought and salt stress tolerance observed...
(a&&"performance"in a&&a.performance&&"function"==typeof a.performance.setResourceTimingBufferSize)a.performance.setResourceTimingBufferSize();!function(){if(BOOMR=a.BOOMR||{},BOOMR.plugins=BOOMR.plugins||{},!BOOMR.plugins.AK){var e=""=="true"?1:0,t="",n="niujgqyxbiaooypocpqq-...