四川泸州医学院附属医院检验科 646000 【摘要】目的：探讨念珠菌细胞壁成分(1-3)-β-D葡聚糖检测的体外影响因素；方法：实验组：将白色假丝酵母菌用正常血浆、无热原蒸馏水、0.9%的生理盐水、0.02%两性霉素B生理盐水和0.75%氟康唑生理盐水稀释为0.5麦氏单位（含白色念珠菌1X107CFU/ml）,对照组：将上述各...
respectively. Ninety crabs were employed and randomly divided into three groups. According to previous study, a volume of 100 μL aliveA. hydrophila(1 × 107CFU mL−1) or lipopolysaccharide (LPS fromEscherichia coli0111:B4, L2630, Sigma Aldrich, USA; 100 µg mL...
solanacearum (1 × 107 cfu/ml), and disease symptoms were checked at 6 dpi. b Estimation of bacterial population in leaves transformed with Agrobacterium containing overexpression vector or no vector (mock treatment) c Chlorophyll content was measured in leaves transformed with Agrobacterium containing...
Col-0 plants were inoculated with Pst AvrRpm1 (5 Â 107 cfu ml À 1). Leaf samples were harvested from areas both inside and immediately neighbouring the infiltrated zone at the indicated time points. Relative expression of MIEL1 (a) and MYB30 (b) in the inoculated (solid line) or...
Caco-2 cells were incubated with 1.5 ml of DMEM without FBS containing either 1 × 107 CFU/ml LGG wild-type or msp1 mutant CMPG10200, 100 ng/ml wild-type, recombinant or TFMS-treated Msp1. DMEM without FBS was used as a negative control. After 16 h incubation at 37°C, cell ...
The growth capacities were assessed every 3 days for 6 or 28 days by determining the colony forming unit (CFU) on 7H10 agar (DIFCO, USA) supplemented with 10% ADC from triplicate tubes of each strain. For starvation cultures, 1 mL of the PBST suspension was added to polypropylene tubes...
We added 2.5% L.P dy-1 (4 × 108 cfu/mL) to the barley flour with seven-times deionized water to ferment at 30 ℃ for 24 h. After centrifugation (8, 000 ×g for 20 min), the supernatant of the fermented liquid was dried with vacuum freeze drier (Marin Christ, Germany) and ...
The bacterial inoculums were adjusted at 0.5 McFarland (1–2) × 108 CFU/ml, using UV–Vis. spectrophotometer at 600 nm. On the other hand, the yeast inoculum was set at 0.5 McFarland (1–5) × 106 CFU/ml. All examined ferrite NPs and DMSO were tested upon different...
In contrast to our co-infected control group displaying a high bacterial load (9 Â 107 CFU per ml), no bacteria were detected in the blood of mice treated with the inhibitor. We also observed a significant reduction in the airway proinflammatory cytokines interleukin (IL)-6 DISCUSSION The ...
For the CFU assays, HPCs were isolated from BM as described in the ‘Haematopoietic-cell and organ preparation’ section. The HPCs were plated (15,000 cells per 35 mm dish) in MethoCult M3231 medium supplemented with 100 ng ml−1 SCF, 10 ng ml−1 GM-CSF, 10 ng ...