Gamma-H2AX is the first step in recruiting and localizing DNA repair proteins. DSBs can be induced by mechanisms such as ionizing radiation or cytotoxic agents and subsequently, gamma-H2AX foci quickly form. These foci represent the DSBs in a 1:1 manner and can...
Wip1 directly dephosphorylates gamma-H2AX and attenuates the DNA damage response. Cancer research 70, 4112–4122 (2010). Article CAS PubMed PubMed Central Google Scholar Freeman, A. K. & Monteiro, A. N. Phosphatases in the cellular response to DNA damage. Cell communication and signaling: ...
gamma-H2AX Antibody (A700-053) in ICC/IF Detection of human gamma-H2AX by immunocytochemistry. Sample: FFPE section of human HeLa cells treated with etoposide. Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody [BLR053F] (Product # A700-053lot 2) used at 1:250. Secondary: HRP...
γ-H2AX as a biomarker of DNA damage induced by ionizing radiation in human peripheral blood lymphocytes and artificial skin Sedelnikova, gamma-H2AX as a biomarker of DNA damage induced by ionizing radiation in human peripheral blood lymphocytes and artificial skin, Advances in ... CE Redon,JS ...
Phosphorylation of the Ser-139 residue of the histone variant H2AX, forming gammaH2AX, is an early cellular response to the induction of DNA double-strand breaks. Detection of this phosphorylation event has emerged as a highly specific and sensitive molecular marker for monitoring DNA damage initiati...
Here we present the methods and the bio-sampling for γ-H2AX detection, γ-H2AX formation in normal cells and animal tissues, in cancer cell lines/tissues and in clinical trials after radiation treatment, alone or in combination with other factors. The purpose of this review is to highlight ...
(2007). gamma-H2AX foci formation in peripheral blood lymphocytes of tumor patients after local radiotherapy to different sites of the body: Dependence on the dose-distribution, irradiated site and time from start of treatment. International Journal of Radiation Biology: Vol. 83, No. 10, pp. 63...
We have investigated the use of the gamma-H2AX assay, reflecting the presence of DNA double-strand breaks (DSBs), as a possible means for identifying individuals who may be intermediate with respect to the extremes of hyper-radiosensitivity phenotypes. In this case, cells were studied from mice...
Gamma-H2AX Flowcytometry 1. Introduction The number of nuclear medicine examinations is increasingly growing, and the principles of radiation protection are important in order to reduce the risk of radiation [1]. Eventually, the best and safest practices will be brought into clinical reality. Wi-Fi...