Primers for nucleic acid amplification in detecting beta-actin and test method using these primers.It is intended to provide novel primers for nucleic acid amplification to be used in detecting mRNA of a housekeeping gene, more particularly, confirming the amplification of 尾-actin or GAPDH. ...
aorta, reverse transcribed (RT) and used in polymerase chain reaction (PCR) amplification with primers specific for NOS II, NOS III and beta-actin. PCR... Philippe Sogni a b,Adrian P.L. Smith a,Adrian Gadano b,... - 《Journal of Hepatology》 被引量: 294发表: 1997年 Inhibition of ...
RDP-39-025 Human GAPDH Primer Pair (25 TESTS)RDP-58-025 Human/Mouse Fas Ligand/TNFSF6 Primer Pair (25 TESTS)RDPC1 Oligo (dT) 12-18 Primers (25 UG)RFBP10 Mouse/Rat FABP1/L-FABP Quantikine ELISA Kit (1 KT)RGM00 Rat GM-CSF Quantikine ELISA Kit (1 KT)RHMTA0 Rhesus Macaque TNF-...
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Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan™ Gene Expression Assays, Custom TaqMan™ Gene Expression Assays, and⁄or Custom TaqMan...
Primers and probes for the in-vitro detection of Human HSV- 1 and HSV-2 virus in a sample comprising nucleotide sequences selected from the group comprising of or any combinations thereof: a nucleic acid molecule that encodes a nucleotide sequence of SEQ ID NO: l and SEQ ID No. 4 or ...
Probe is labeled with 6FAM™ dye - MGB and the primers are not limited. Can be used for singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan™ Gene Expression Assays, Custom TaqMan™ Gene Expression Assays, and⁄or Custom TaqMan™ ...
qRT-PCR-based expression assays were performed using primers designed for gamma-actin, beta-actin, GAPDH, and 18S genes. We calculated the amplification efficiency, specificity, and stability using geNorm, NormFinder, BestKeeper, and the 螖Ct comparative method. We obtained linear regr...
Disclosed herein are primers and probes related to the detection of beta actin [(human)] via nucleic acid amplification testing (NAAT), for example to amplify and determine the presence of 尾-actin present in test samples. Specifically, the present disclosure describes primers and probes that ...
A strong correlation was observed between cell numbers and Ct values among olive flounder, sevenband grouper, rock bream, rock fish, rainbow trout and fish cell lines (FHM, EPC, SSN-1, BF-2 and GF), suggesting that cell number can be counted by qPCR assay using the universal primers ...