Mutational analysis of the mouse $\\beta$-globin poly(A) signal function in COS cells revealed a striking bipartite structure of the downstream element, located downstream of the poly(A) addition site, and one novel cleavage site determinant for cleavage site selection. From analysis of the ...
Furthermore, the presence of this efficient polyadenylation signal resulted in a 1.4- to 2.5-fold increase in the fraction of virus-specific RNAs that were polyadenylated. Most importantly, termination of transcription by RNA polymerase II on ins 5 DNA was also increased compared with wild-type ...
Full details of the exon/intron lengths, location of the putative poly-A addition site (AATAAA) and the lengths of the coding domains with the predicted encoded polypeptide for each predicted gene are given in Table 1. Figure 5B shows the predictions for some of the well-characterised protein...
Mutational analysis of the mouse $\\beta$-globin poly(A) signal function in COS cells revealed a striking bipartite structure of the downstream element, lo... Chen,Jingshan 被引量: 0发表: 1994年 Bipartite structure of the downstream element of the mouse beta globin (major) poly(A) signal ...
The globin mRNAs containing between 30 and 40 polyadenylate residues can be separated from thos mRNAs containing longer poly(A) regions by Millipore filter binding. The molecular weights of the alpha-and beta-globin mRNAs containing this size class of poly(A) have beed determined by lectrophoresis...
Alpha-thalassaemia caused by a poly(A) site mutation reveals that transcriptional termination is linked to 3' end processing in the human alpha 2 globin gene. We have investigated the process of transcriptional termination in the duplicated human alpha globin genes which lie 4 kb apart on chromoso...
We found that segments located about 400 bp downstream from the poly(A) site of both the chicken and duck βA-globin genes (and about 1.5 kb upstream from the embryonic ε-globin gene) stimulate transcription of the test genes about five-fold. In chicken essentially the same segment was ...
The downstream region of the mouse beta (major) globin poly(A) signal was mutated and analyzed for function in transfected COS cells. From analysis of unidirectional Bal31 deletions, the 3' boundary of the downstream element was defined as +22 (22 nucleotides downstream from the cleavage site)...
β-mutations at CD 19, IVS1-1 (G-T), IVS1-5, poly A, and hemoglobin E caused 76% of the hemoglobin disorders in the Malays. The Filipino 45-kb deletion caused 73.3% of bthalassemia in the Orang Asli. Using genomic sequencing, the rare Chinese β-mutation at CD 43 (G-T) was ...
Two β -globin DNA elements were required to effect efficient termination: an upstream sequence that includes two poly(A) addition signals and a downstream region previously shown to be where RNA synthesis stops. The role of poly(A) addition in termination was established by introduction of ...