Mutational analysis of the mouse $\\beta$-globin poly(A) signal function in COS cells revealed a striking bipartite structure of the downstream element, located downstream of the poly(A) addition site, and one novel cleavage site determinant for cleavage site selection. From analysis of the ...
Furthermore, the presence of this efficient polyadenylation signal resulted in a 1.4- to 2.5-fold increase in the fraction of virus-specific RNAs that were polyadenylated. Most importantly, termination of transcription by RNA polymerase II on ins 5 DNA was also increased compared with wild-type ...
Full details of the exon/intron lengths, location of the putative poly-A addition site (AATAAA) and the lengths of the coding domains with the predicted encoded polypeptide for each predicted gene are given in Table 1. Figure 5B shows the predictions for some of the well-characterised protein...
The downstream region of the mouse beta (major) globin poly(A) signal was mutated and analyzed for function in transfected COS cells. From analysis of unidirectional Bal31 deletions, the 3' boundary of the downstream element was defined as +22 (22 nucleotides downstream from the cleavage site)...
The molecular weights of the alpha-and beta-globin mRNAs containing this size class of poly(A) have beed determined by lectrophoresis on 3.7% polyacrylamide gels in the presence of 99% formamide. Because the number of adenylic acid residues in these mRNAs is known, the number of non-poly(...
Alpha-thalassaemia caused by a poly(A) site mutation reveals that transcriptional termination is linked to 3' end processing in the human alpha 2 globin gene. We have investigated the process of transcriptional termination in the duplicated human alpha globin genes which lie 4 kb apart on chromoso...
Recently, in vivo analysis of transcriptional termination for the human beta-globin gene revealed a new phenomenon--co-transcriptional cleavage (CoTC). This primary cleavage event within beta-globin pre-messenger RNA, downstream of the poly(A) site, is critical for efficient transcriptional ...
A possible protecting function for the poly(A) sequence is suggested by the fact that molecules with very short poly(A) segments were cleaved preferentially in this region. The G residues in the 5′ non-coding region were inaccessible to RNase T 1. A highly sensitive site adjacent to the ...
Mutational analysis of the mouse $\\beta$-globin poly(A) signal function in COS cells revealed a striking bipartite structure of the downstream element, lo... Chen,Jingshan 被引量: 0发表: 1994年 Bipartite structure of the downstream element of the mouse beta globin (major) poly(A) signal ...
1988. A rabbit beta-globin polyadenylation signal directs efficient termination of transcription of polyomavirus DNA. EMBO J 7:2515-2522.Lanoix, J., and N. H. Acheson. 1988. A rabbit 3-globin polyad- enylation signal directs efficient termination of transcription of polyoma virus DNA. EMBO ...