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Other reporter genes are based on green fluorescent protein (GFP; ref. 5 ) or the Escherichia coli lacZ gene, which encodes for 尾-galactosidase for X-gal staining ( 4 , 6 ). GFP-expressing cells can be easily visualized under an ultraviolet microscope. The main advantage is that GFP ...
Bluo-Gal may be particularly useful in some histochemical applications because the cleavage product precipitates as fine biréfringent crystals that emit a strong optical signal (yellow), allowing enhanced resolution of staining patterns under a polarized light microscope (106). βGal samples dot-blotted...
1531303 Lactase IMPERIAL CHEMICAL INDUSTRIES Ltd 31 Aug 1977 [14 Sept 1976] 38002/76 Heading C3H [Also in Division C2] A beta-galactosidase (lactase) enzyme is prepared by culturing a micro-organism belonging to the genus of which the micro-organism designated LT-2 (NCIB No. 11259) is a...
Applications Reported: Purified Anti-beta galactosidase fusion proteins poly has been reported for use in immunoblotting (WB), and immunohistochemical staining.Applications Tested: Purified Anti-beta galactosidase fusion proteins poly has been tested by immunoblotting (WB). (1:400 starting dilution...
The SA-β-gal staining results of frozen tissue sections showed a significant increase in staining in the D-gal aging model group. Eudesmin exhibited a significant protective effect against pancreatic cell aging (Fig. 4A). Similarly, the immunofluorescence results of the aging-related cell cycle ...
X-gal staining was used to check expression of β-gal. Results β-gal was expressed strongly and well-distributed in tumor in the first group, and stained blue in the liver, spleen, lung and kidney, but much weaker than that of tumor. In the second group, β-gal was expressed in the...
Ricinus Communis Agglutinin -I staining indicated generous increase of Galβ 1→4GlcNAc groups during apoptosis. Further study showed that the 7721 hepatocarcinoma cells transiently transfected with β 1,4GT1 were more susceptible to the apoptosis induced by cycloheximide. The increased susceptibility was...
Chromosomes stained with certain dyes, commonly quinacrine (Q banding) or Giemsa (G banding), show a pattern of transverse bands of light and heavy staining that is characteristic for the individual chromosome. The basis of the differential staining, which is the same in most tissues, is not ...
Linke R . Congo red staining of amyloid: improvements and practical guide for a more precise diagnosis of amyloid and the different amyloidoses. In: Uversky V, Fink A (ed). Protein Misfolding, Aggregation, and Conformational Diseases. Springer: USA, 2006, p 239–276. Book Google Scholar ...