To investigate the activity of senescence-associated beta-galactosidase (SA-beta-GAL) in the hippocampus of aging rats. Hippocampi of 6-, 18-, and 24-month-old rats were observed by histochemical staining for SA-beta-GAL and cytochemical staining for SA-beta-GAL in cultured hippocampal neurons...
The cytochemical staining of senescence associated β-galactosidase(SA-β-GAL)was applied to cultured neurons for 6、12 and 20 days. 方法:利用SD新生鼠进行海马神经元原代培养,分别取培养6、12和20d的神经元进行衰老相关β半乳糖苷酶(SA-β-GAL)的细胞化学染色。4...
The basic colorimetric assay described here is the simplest and least expensive assay for quantifying beta-gal activity. The cells are lysed and, after determining the total protein concentration in the extracts, an aliquot of the extract is mixed with the reaction substrate, O-nitrophenyl-beta-D...
Cell lines and primary cultures from several teleost tissues and species were stained for senescence-associated beta-galactosidase (SA beta-Gal), revealing four general outcomes. (1) For long-standing fish cell lines that can be considered immortal, little or no SA beta-Gal staining was observed...
SA-beta-Gal staining was found in a subset of the chondrocytes close to the lesion site of mild, moderate and severe damaged knee OA cartilage. The percentage of SA-beta-Gal-positive chondrocytes in articular cartilage was 0% in controls, 13.00卤5.77% in mild lesions, 31.65卤6.91% in ...
The in-situ SERS readout strategy is proved to be necessary and feasible to improve the reliability of X-gal staining assay through shortening the time to a few hours. Moreover, its application was also preliminarily evaluated to analyse individual cells and tissues, which showed the well ...
SA-β-Gal positive cells and senescence-associated heterochromatic foci (SAHF) were determined by assessing blue color formation of substrate, X-gal inside cells and DAPI staining, respectively. Cell cycle and hypodiploid cell formation were assessed by flow cytometry analysis. CREB phosphorylation and...
Except for the alveolar macrophages, which are enriched with lysosomal �-galactosidase =-=(21)-=-, the SA �-gal staining of alveolar epithelial cells was almost completely absent in the lungs of mice that were not exposed to CS (Figure 6A), whereas alveolar epithelial cells that stained...
cell differentiation and/or apoptosis. We, therefore, set out to (a) investigate the presence of enhanced β-gal-pH6 during the development of the mouse olfactory epithelium during early postnatal life and (b) identify the populations of cells that exhibit high levels of β-gal-pH6 staining....
The cytochemical staining of senescence associated β-galactosidase(SA-β-GAL)was applied to cultured neurons for 6、12 and 20 days. 方法:利用SD新生鼠进行海马神经元原代培养,分别取培养6、12和20d的神经元进行衰老相关β半乳糖苷酶(SA-β-GAL)的细胞化学染色。4...