(1:1000; Roche, Basel, Switzerland), rabbit polyclonal anti-hepatocyte nuclear factor 1β (Hnf1β; 1:50; Santa Cruz Biotechnology, Dallas, TX, USA), rabbit monoclonal anti-Nkx6.1 (1:400; Abcam), chick polyclonal anti-β-galactosidase (β-gal; 1:100; Abcam), and mouse monoclonal anti-...
senescence, such as activation of a DNA damage response (DDR), an enlarged and flattened morphology, cyclin-dependent kinase inhibitor (CDKI)-dependent cell-cycle arrest, resistance to apoptosis, and increased activity of senescence-associated β-galactosidase (SA-βgal) (He and Sharpless, 2017)....
A typical IgG antibody is composed of two light and two heavy chains that are associated with each other to form three major domains connected through a flexible hinge region: the two identical antigen-binding (Fab) regions and the constant (Fc) region. It is noted that there are heterogeneou...
Beta-galactosidase fusion protein with HIV-1 Tat PTD mediates the rapid spread of beta-galactosidase in the liver, heart, and other tissues of mice [63]. The spread of beta-galactosidase is not observed when only enzyme is administered [26,27]. Furthermore, previously we have shown that PTD...
macquariensis D50 were constructed and used to clone and express a gene-encoding cold-active β-d-galactosidase of Paracoccus sp. 32d. The yield was 1150 U/L of recombinant yeast culture. Recombinant D. macquariensis D50 strains were mitotically stable under both selective and non-selective ...
Utilized amounts of PKC vary from one study to another [18], most likely due to lack of proper systematic documentation as well as addition of different kinds of enzymes including NSPase such as mannanase alone or, for example, together with xylanases, proteases and galactosidases [20] in ...
Topoisomerase IIIβ (Top3β), the only dual-activity topoisomerase in mammals that can change topology of both DNA and RNA, is known to be associated with neurodevelopment and mental dysfunction in humans. However, there is no report showing clear associ