PROBLEM TO BE SOLVED: To obtain a new thermally stabler α-galactosidase protein derived from a strictly anaerobic thermophilic bacterium, and to provide a gene encoding the protein, a recombinant vector containing the gene, a transformant containing the recombinant vector, and to provide a method...
Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions We report yeast/Escherichia coli shuttle vectors suitable for fusing yeast promoter and coding sequences to the lacZgene of E. coli. The vectors contain a ......
The first gene (named aga) codes for an enzyme with an alpha-galactosidase activity. The second gene (named galR) codes for a transcriptional regulator which would act as a regulator of aga. When present in a lactic acid bacterium such as , this DNA fragment can modify the sugar ...
Broad range activity High activity in the condition of pH3.5~6,37°C Higher purity Employing advanced international production strain and standard process to ensure product quality Higher activity Utilizing galactosidase gene with high specific activity and top liquid fermentation to keep product high act...
Genetic analysis of the alpha-galactosidase A gene was performed in 22 hemizygous males and 34 females. One of the female patients with severe disease phenotype showed homozygosity for the recurrent c.644A>G mutation due to parental consanguinity. The c.644A>G mutation that has previously been ...
Gene ID (NCBI) 2717 RRID AB_2881520 Conjugate Unconjugated Form Liquid Purification Method Protein A purification UNIPROT ID P06280 Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Storage Conditions Store at -20°C. Stable for one year after shipment. Aliquoting is unnecess...
Reports the generation of alpha-Gal A deficient mice by gene targeting and an analysis of the resulting phenotype. Gene targeting and generation of mutant ... Ohshima,Toshio,Murray,... - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: 391发表: 199...
Fabry disease is an X-linked disorder; the GLA gene is located on the long arm of the X chromosome. Consequently, female patients with the disease are heterozygous and are afected to varying degrees, ranging from seemingly asymptomatic to severe. Clinical presentation varies considerably, even am...
Gene ID (NCBI) 2717 RRID AB_2883263 Conjugate CoraLite® Plus 488 Fluorescent Dye Excitation/Emission Maxima Wavelengths 493 nm / 522 nm Form Liquid Purification Method Protein A purification UNIPROT ID P06280 Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. Storage...
The alpha-galactosidase A gene has been mapped to the region q22.1 of the X chromosome.5–8 When mutated, a progressive accumulation of a glycolipid called globotriaosylceramide (Gb3) affects mainly endothelial and vascular smooth muscle cells and causes damage to many major organs1 including ...