PURPOSE: To obtain a new gene coding a β-galactosidase having a specific amino acid sequence and originated from Aspergillus oryzae, and giving a transformant highly expressing the above-mentioned enzyme useful for converting lactose into useful saccharides outside the cell.ITO YOSHIYUKI...
摘要: PURPOSE: To obtain a new gene coding a β-galactosidase having a specific amino acid sequence and originated from Aspergillus oryzae, and giving a transformant highly expressing the above-mentioned enzyme useful for converting lactose into useful saccharides outside the cell....
clones. This enzyme can be split in two peptides, LacZα and LacZΩ, neither of which is active by itself but when both are present together, spontaneously reassemble into a functional enzyme. This property is exploited in many cloning vectors where the presence of the lacZα gene in a pla...
英文: Rapid Detection of Beta-galactosidase Activity by Test Paper中文: 用试纸法快速检测β-半乳糖苷酶活性的研究 英文: Cloning and expression of the beta-galactosidase gene of Paenibacillus sp.K1 in E.coli中文: Paenibacillus sp.K1 β-半乳糖苷酶基因的克隆及在大肠杆菌中的表达 ...
A gene encoding a third 伪-galactosidase (AglB) fromAspergillus niger has been cloned and sequenced. The gene consists of an open reading frame of 1,750 bp containing six introns. The gene encodes a protein of 443 amino acids which conta... Ronald,P.,de,... - 《Appl.environ.microbiol...
1999. Six novel beta-galactosidase gene mutations in Brazilian patients with GM1-gangliosidosis. Hum Mutat 13:401-409.Severini MH et al. (1999) Six novel beta-galactosidase gene mutations in Brazilian patients with GM1-gangliosidosis. Hum Mutat. 13(5):401-409....
DNA FRAGMENT CONTAINING BETA-GALACTOSIDASE GENE AND PLASMID CONTAINING THE SAME DNA FRAGMENT INTEGRATED THEREINTO 来自 百度文库 喜欢 0 阅读量: 9 申请(专利)号: JP特願平3-335904 申请日期: 19911127 公开/公告号: JP特開平5-146296A 公开/公告日期: 19930615 ...
The GLB1 gene encodes beta-galactosidase-1 (EC 3.2.1.23),a lysosomal hydrolase that cleaves the terminal beta-galactose from ganglioside substrates and other glycoconjugates (Yoshida et al.,1991 [PubMed 1907800]). Beta-galactosidase also occurs in
A series of nonsense mutants which map throughout the β-galactosidase structural gene, and several missense and deletion mutants, were analysed for antigenic activity with purified antiserum produced from native β-galactosidase. Quantitative analyses by the Ouchterlony test, by inhibition studies, and...
This result suggests that a short hsp70 gene segment consisting of 195 base pairs of upstream sequence and a complete RNA leader region contain all the information required for the induced synthesis of proteins during heat shock. 展开 关键词: heat stress, cells, beta-galactosidase, Drosophila ...