Using timed CVs, the enzyme kinetics of β-Gal versus 4-aminophenyl-β-D-galactopyranoside (PAPG) was estimated. The detection limit for β-Gal was 40 ng ml using the timed quick CV method. KM was found to be 18.7 ± 0.7 M, and Vmax 10.1 ± 0.6 mol min mg, for β-Gal with PA...
Beta-galactosidase: An essential enzyme Overview Beta-galactosidase, also known as beta-Gal or GLB1, is an essential enzyme that hydrolyzes beta-galactosides into monosaccharides. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. ...
Beta-galactosidase is an exoglycosidase which hydrolyzes the β-glycosidic bond formed between agalactose and its organic moiety. It may also cleave fucosides and arabinosides but with much lower efficiency. It is an essential enzyme in the human body. Deficiencies in the protein can result ingal...
Beta-galactosidase: An essential enzymeOverview Beta-galactosidase, also known as beta-Gal or GLB1, is an essential enzyme that hydrolyzes beta-galactosides into monosaccharides. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. Antibody Type ...
Beta-galactosidase gene mutations affecting the lysosomal enzyme and the elastin-binding protein in GM1-gangliosidosis patients with cardiac involvement. Hum Mutat 2000;15(4):354-366. [PubMed: 10737981]Morrone A, Bardelli T, Donati MA, et al. Beta-galactosidase gene mutations affecting the ...
[27] with the in situ Cell Death Detection Kit, POD (Roche Molecular Biochemicals, Mannheim, Germany). The TUNEL technique was performed as described in Francisco-Morcillo et al. (2004) [46]. No stained nuclei were observed in control sections in which the enzyme TdT had been omitted from...
Enzyme linked immunosorbent assay (ELISA) 1:100～1000(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.相关搜索:Beta ...
Process for producing the enzyme -galactosidase A process for the production of the enzyme lactase or -galactosidase by yeasts of the genus Kluyveromyces cultured in the solid state on granules of maize ear heart, and others having similar properties, moistened with concentrated milk serum which make...
Lac-z-gene produces beta-galactosidase enzyme, which is responsible for the colour of colonies. en.wikipedia.org These assays make use of a sugar linked to a dye which, when acted on by the enzyme beta-galactosidase, produces a characteristic color. ...
This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene have been associated with GM1-gangliosidosis and Morquio B syndrome. Diseases associated with GLB1 include Gm1-Gangliosidosis, Type I and Gm...